|
| Status |
Public on Jun 10, 2016 |
| Title |
low CO2 (rep 3) |
| Sample type |
RNA |
| |
|
| Source name |
Leaves
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
treatment: low CO2
age: 6 weeks
|
| Treatment protocol |
After 6 weeks plants were transferred to low CO2 conditions (100 ppm) during 24 h or kept at 400 ppm CO2 (control condition).
|
| Growth protocol |
Plants were grown in soil under normal (400 ppm) CO2 conditions under a short day light regime.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For each replicate plant leaf material was pooled from 4-5 individual plants and total RNA was isolated using Trizol Reagent (Life Technologies, Gaithersburg, USA).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 5 microg total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C and at 60 rounds/min. The GeneChips were washed and stained in the Affymetrix Fluidics Station 400 and 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
| Description |
After 6 weeks plants were transferred to low CO2 conditions (100 ppm) during 24 h or kept at 400 ppm CO2 (control condition).
|
| Data processing |
Normalised using GCRMA using an updated probe annotation (TAIRGv18.0.0)
|
| |
|
| Submission date |
Apr 11, 2016 |
| Last update date |
Jun 10, 2016 |
| Contact name |
Patrick Willems |
| E-mail(s) |
[email protected]
|
| Organization name |
Ghent University
|
| Department |
PSB
|
| Street address |
Technologiepark 927
|
| City |
Gent |
| ZIP/Postal code |
9052 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL21063 |
| Series (1) |
| GSE80158 |
Photorespiratory stress low CO2 conditions |
|
