|
| Status |
Public on Dec 07, 2016 |
| Title |
Small RNAs from ovaries of H111 transgenic strain |
| Sample type |
SRA |
| |
|
| Source name |
ovaries
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: ovaries
strain: H111
|
| Growth protocol |
Flies were grown according to standard protocols.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by Trizol (Invitrogene) and size-fractioned by PAGE (19-29 nt)
Libraries of small RNAs 19-29 nt were constructed as previously described in (Muerdter et al. 2012)
|
| |
|
| Library strategy |
ncRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
The reads with clipped 3'-adapter were passed through length (>18 nt), quality (80% of nt should contain >=20 Phred score) and artefacts filters, and mapped by bowtie to dm3 genome assembly with requiring of perfect mathcing.
Genome_build: dm3
Supplementary_files_format_and_content: Tab-delimited files include the frequencies of clipped read occurrences in each library.
|
| |
|
| Submission date |
Feb 20, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Sergei Ryazansky |
| E-mail(s) |
[email protected]
|
| Phone |
+7-499-196-81-73
|
| Organization name |
Institute of Molecular Genetics
|
| Department |
Department of Molecular Genetics of Cell
|
| Street address |
Kurchatov sq., 2
|
| City |
Moscow |
| ZIP/Postal code |
123182 |
| Country |
Russia |
| |
|
| Platform ID |
GPL13304 |
| Series (1) |
| GSE78135 |
Telomeric retrotransposon HeT-A contains a bidirectional promoter that initiates divergent transcription of piRNA precursors in Drosophila germline |
|
| Relations |
| BioSample |
SAMN04505633 |
| SRA |
SRX1595487 |
