|
| Status |
Public on Jan 01, 2017 |
| Title |
PGN-319_1 Data from Strain KDP315 |
| Sample type |
RNA |
| |
|
| Source name |
Microorganism frequently isolated from lesion of chronic periodontitis
|
| Organism |
Porphyromonas gingivalis ATCC 33277 |
| Characteristics |
strain: KDP315
genotype/variation: PGN_0319:: ermF ermAM, Emr
|
| Treatment protocol |
None
|
| Growth protocol |
P. gingivalis cells grown to an OD660 of 0.6-0.8 in brain heart infusion broth (Becton Dickinson Sparks, MD) supplemented with 0.5% yeast extract, 5 µg/ml menadione and 0.5 µg /ml hemin at 37°C under anaerobic condition.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol reagent (Invitorogen, Carlsbad, CA)
|
| Label |
Cy3
|
| Label protocol |
Cy3 label with Input Quick-Amp WT Labeling Kit (Agilent Technologies, Santa Clara, CA)
|
| |
|
| Hybridization protocol |
1. Fragmentation of Cy3 labeled cRNA was carried out at 60°C for 30 min. 2. Stop fragmentation. 3. Hybridization was performed at 65°C for 17 h with agitation in Hybrioven.
|
| Scan protocol |
Scanned by Agilent Technologies Microarray Scanner.
|
| Description |
Deficient mutant of PGN-0319 constructed from P. gingivalis ATCC 33277.
|
| Data processing |
Normalization (Per Chip: Normalize to 75 percentile) was used GeneSpring GX software.
|
| |
|
| Submission date |
Feb 18, 2016 |
| Last update date |
Jan 01, 2017 |
| Contact name |
Kazuyuki Ishihara |
| Organization name |
Tokyo Dental College
|
| Department |
Microbiolgy
|
| Street address |
Misakicho 2-1-14
|
| City |
Chiyoda-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
101-0061 |
| Country |
Japan |
| |
|
| Platform ID |
GPL21477 |
| Series (1) |
| GSE78048 |
Role of an extracytoplasmic function sigma factor, PGN_0319, in hemin utilization by Porphyromonas gingivalis |
|
