|
| Status |
Public on Aug 31, 2016 |
| Title |
HBEC3_ZEB1.1 |
| Sample type |
RNA |
| |
|
| Source name |
HBEC3-KTZEB1.1
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Human bronchial epithelial cell (HBEC) immortalized with cdk4 and hTERT
sh-p53: no
krasv12: no
cmyc: no
zeb1: yes
cd24hi (facs): no
cd44hi (facs): no
growth media: KSFM
age: 65
gender: F
smoker: yes
|
| Growth protocol |
Cells were grown in adherent conditions and cell pellets were harvested when cells were ~70% confluent.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using RNeasy mini kit (QIAGEN, Valencia, CA) and quality was analyzed using an Agilent 2100 Bioanalyzer (Agilent Technologies Inc., Santa Clara, CA).
|
| Label |
Biotin
|
| Label protocol |
500 ng of total RNA was used to label the cRNA probes by Ambion Illumina TotalPrep RNA Amplification kit (Cat# IL1791)
|
| |
|
| Hybridization protocol |
1.5 ug of the amplified and labeled cRNA probes was hybridized to the chip overnight at 58 degree C, then washed, blocked and detected by streptavidin-Cy3 per manufacturer's protocol.
|
| Scan protocol |
The chips were scanned by Illumina iScan system.
|
| Description |
HBEC3-KTZEB1.1
|
| Data processing |
Bead-level data were processed with the MBCB algorithm (Ding et al, Nucl Acids Res, 36:e58, 2008), which is a background-correction and summarization method, then quantile-normalized and log2-transformed
|
| |
|
| Submission date |
Feb 15, 2016 |
| Last update date |
Aug 31, 2016 |
| Contact name |
Luc Girard |
| E-mail(s) |
[email protected]
|
| Organization name |
UT Southwestern Medical Center
|
| Department |
Hamon Center for Therapeutic Oncology Research
|
| Lab |
NB8.114A
|
| Street address |
5323 Harry Hines Blvd
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390-8593 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE77925 |
Defining the role of ZEB1 in the pathogenesis of lung cancer |
|
