|
| Status |
Public on Feb 06, 2017 |
| Title |
19b |
| Sample type |
SRA |
| |
|
| Source name |
DN1
|
| Organism |
Drosophila melanogaster |
| Characteristics |
timepoint: ZT19
tissue: neurons
type: DN1
|
| Growth protocol |
Drosophila were entrained for 2-4 days in LD cycles and then collected at specified timepoints.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Neurons were lysed and RNA extracted and purified using oligo-dT. cDNA was made using primer (random and dT mixed) with T7 promoters. cDNA was used as a template for 1 round of linear amplification using T7-polymerase. Resutling cRNa used as input for RNA TruSEQ v2 library construction.
Illumina TruSeq v2
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
DN1_19b
|
| Data processing |
Aligned to dm3 using tophat2.0
Gene expression quantified using End Sequencing Analysis Tool Kit
Cycling gene expression identified using F24 analysis and JTK cycle (both publically available).
Genome_build: Drosophila dm3
Supplementary_files_format_and_content: processed files list gene expression quantification output from ESAT by transcript ID.
|
| |
|
| Submission date |
Feb 01, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Katharine Abruzzi |
| E-mail(s) |
[email protected]
|
| Phone |
7817363161
|
| Organization name |
HHMI; Brandeis University
|
| Department |
Biology
|
| Lab |
Rosbash
|
| Street address |
415 South St.
|
| City |
Waltham |
| State/province |
MA |
| ZIP/Postal code |
02478 |
| Country |
USA |
| |
|
| Platform ID |
GPL13304 |
| Series (1) |
| GSE77451 |
RNA-seq analysis of Drosophila clock and non-clock neurons reveals neuron-specific cycling and novel candidate neuropeptides |
|
| Relations |
| Reanalyzed by |
GSM3278473 |
| BioSample |
SAMN04450936 |
| SRA |
SRX1556145 |
