|
| Status |
Public on Aug 10, 2016 |
| Title |
1025 |
| Sample type |
RNA |
| |
|
| Source name |
Wildtype for +1 line /Vehicle
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: F344
genotype/variation: Wildtype for +1
tissue: liver
treatment: Vehicle
|
| Treatment protocol |
Gavage of CDDO-Im (30 umol/kg body weight, 6 hours)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from whole livers using Sepazol-RNA I Suger G (Nacalai Tesque).
|
| Label |
Cy3
|
| Label protocol |
500 ng of total RNA was amplified and labeled using the Agilent Low RNA Input Fluorescent Linear Amplification Kit according to the manufacturer's protocol.
|
| |
|
| Hybridization protocol |
1.65 ug of Cy3-labeled cRNA were mixed and fragmented using the Agilent In Situ Hybridization Kit protocol. Hybridizations were performed for 16 hours in a rotating hybridization oven according to the Agilent 60-mer oligo microarray processing protocol prior to washing.
|
| Scan protocol |
Slides were scanned with an Agilent DNA Microarray Scanner model G2539.
|
| Description |
Gene expression in Vehicle-treated Wildtype rat
|
| Data processing |
Data was obtained using the Agilent Feature Extraction software (v10.1), using default for all parameters. The data were analyzed using GeneSpring GX software (v11.5) (Agilent).
|
| |
|
| Submission date |
Jan 29, 2016 |
| Last update date |
Aug 10, 2016 |
| Contact name |
Keiko Taguchi |
| E-mail(s) |
[email protected]
|
| Organization name |
Tohoku University
|
| Department |
Graduate School of Medicine
|
| Lab |
Medical Biochemistry
|
| Street address |
2-1 Seiryo-cho, Aoba
|
| City |
Sendai |
| State/province |
Miyagi |
| ZIP/Postal code |
980-8575 |
| Country |
Japan |
| |
|
| Platform ID |
GPL14746 |
| Series (1) |
| GSE77377 |
Comparison of gene expression profiles of wildtype and two lines of Nrf2 knockout rat livers with or without CDDO-Im treatment |
|
