|
| Status |
Public on May 25, 2016 |
| Title |
Ly-6Chi TremL4+ mono |
| Sample type |
RNA |
| |
|
| Source name |
WT C57Bl/6
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6
gender: Female
genotype: WT
cell type: Ly-6Chi TremL4+ monocyte
treatment: none
|
| Treatment protocol |
Mice were analyzed at steady state
|
| Growth protocol |
Primary peripheral blood leukocytes were collected from 10 WT C57Bl/6 females
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Monocytes were sorted as: Ter-119– CD45.2+ MHC-IIlo Ly-6G– CD115+. Monocyte subsets were defined as Ly-6ChiTremL4–, Ly-6ChiTremL4+ and Ly-6Clo TremL4+
|
| Label |
biotin
|
| Label protocol |
Ambion WT Expression kit, Genechip WT Terminal Labeling and Controls Kit
|
| |
|
| Hybridization protocol |
Hybed onto Mouse Gene 1.0 ST Arrays (Affymetrix)
|
| Scan protocol |
Standard Affymetrix protocol
|
| Data processing |
Data was processed using RMA summarization and quartile normalization using Arraystar ver. 5
|
| |
|
| Submission date |
Nov 14, 2015 |
| Last update date |
May 25, 2016 |
| Contact name |
Carlos Gustavo Briseno |
| Organization name |
Washington University School of Medicine
|
| Street address |
660 S Euclid
|
| City |
Saint Louis |
| State/province |
Missouri |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE75013 |
Microarray expression data from circulating blood monocytes |
| GSE75015 |
Distinct transcriptional programs control cross-presentation in classical- and monocyte-derived dendritic cells |
|
