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GEO help: Mouse over screen elements for information. |
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| Status |
Public on May 25, 2016 |
| Title |
Microarray expression data from circulating blood monocytes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Cross-presentation of cell-associated antigens is carried out by classical DCs (cDCs) and monocyte-derived DCs (Mo-DCs), but whether a similar or distinct program exists for this process is unknown. In examining this issue, we discovered that only Ly-6ChiTremL4– monocytes, but not Ly-6ChiTremL4+ monocytes, can differentiate into Zbtb46+ Mo-DCs in response to GM-CSF and IL-4. However, Ly-6ChiTremL4+ monocytes were committed to Nur77-dependent development of Ly-6CloTremL4+ monocytes. Further, differentiation of monocytes with GM-CSF required addition of IL-4 to generate Zbtb46+ Mo-DCs that cross-presented as efficiently as CD24+ cDCs, which was accompanied by increased Batf3 and Irf4 expression. Unlike cDCs, Mo-DCs required only IRF4, and not Batf3, for cross-presentation. Further, Irf4–/– monocytes failed to develop into Zbtb46+ Mo-DCs, and instead developed into macrophages. Thus, cDCs and Mo-DCs use distinct transcriptional programs for cross-presentation that may drive different antigen-processing pathways. These differences may influence development of therapeutic DC vaccines based on Mo-DCs.
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| Overall design |
Analysis of Ly-6Chi TremL4– , Ly-6Chi TremL4+ and Ly-6Clo TremL4+ circulating blood monocytes
Peripheral blood was pooled from 10 WT C57Bl/6 females and monocyte subsets were sorted to >95% purity on a FacsAria Fusion
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| Contributor(s) |
Haldar M, Murphy KM |
| Citation(s) |
27264183 |
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| Submission date |
Nov 14, 2015 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Carlos Gustavo Briseno |
| Organization name |
Washington University School of Medicine
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| Street address |
660 S Euclid
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| City |
Saint Louis |
| State/province |
Missouri |
| ZIP/Postal code |
63110 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE75015 |
Distinct transcriptional programs control cross-presentation in classical- and monocyte-derived dendritic cells |
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| Relations |
| BioProject |
PRJNA302204 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE75013_RAW.tar |
10.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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