|
| Status |
Public on Nov 07, 2015 |
| Title |
VSMCs in Calcification media for 3 days rep.1 / VSMCs in Growth media rep.1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Rat VSMCs in Growth media rep.1
|
| Organism |
Rattus norvegicus |
| Characteristics |
gender: male
age: 6 weeks
tissue: Aorta
strain: Sprague-Dawley 400
|
| Treatment protocol |
Calcification was induced in VSMCs with inorganic phosphate (2mmole/L).
|
| Growth protocol |
VSMCs were grown in 10% FBS in DMEM supplemented with antibiotics at 37C, 5 %CO2, >80 %humidity.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol reagent following manufacturer's protocol. Quality was monitored using Agilent 2100 Bioanalyzer.
|
| Label |
Cy3
|
| Label protocol |
Agilent’s Agilent’s Low RNA Input Linear Amplification kit PLUS was used for labeling.
|
| |
|
| Channel 2 |
| Source name |
Rat VSMCs in Calcification media for 3 days rep.1
|
| Organism |
Rattus norvegicus |
| Characteristics |
gender: male
age: 6 weeks
tissue: Aorta
strain: Sprague-Dawley 400
|
| Treatment protocol |
Calcification was induced in VSMCs with inorganic phosphate (2mmole/L).
|
| Growth protocol |
VSMCs were grown in 10% FBS in DMEM supplemented with antibiotics at 37C, 5 %CO2, >80 %humidity.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol reagent following manufacturer's protocol. Quality was monitored using Agilent 2100 Bioanalyzer.
|
| Label |
Cy5
|
| Label protocol |
Agilent’s Agilent’s Low RNA Input Linear Amplification kit PLUS was used for labeling.
|
| |
|
| |
| Hybridization protocol |
Agilent’s Gene Expression Hybridization Kit was used for labeling hybridization.
|
| Scan protocol |
Agilent’s DNA microarray scanner and Feature Extraction Software was used for scaning and data acquisition.
|
| Data processing |
Agilent Feature Extraction Software (v 8.5.1.1) was used for background subtraction, and GeneSpringGX v7.3 Software was used for LOWESS normalization.
Reliable genes (flag-out in 2 of 4 arrays ) 18,112 genes selected
|
| |
|
| Submission date |
Nov 06, 2015 |
| Last update date |
Nov 07, 2015 |
| Contact name |
Nakwon Choe |
| E-mail(s) |
[email protected]
|
| Organization name |
Chonnam Univ. Med. Sch.
|
| Department |
Pharmacology
|
| Street address |
Baekseo-ro 160
|
| City |
Hwasun |
| State/province |
Seoyang-ro 322 |
| ZIP/Postal code |
58128 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL14746 |
| Series (1) |
| GSE74755 |
mRNA expression profile in vascular smooth muscle cells (VSMCs) after inducing calcification |
|
