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| Status |
Public on Sep 01, 2016 |
| Title |
High-dose group |
| Sample type |
RNA |
| |
|
| Source name |
Kidneys_high-dose_HBMP
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| Organism |
Rattus norvegicus |
| Characteristics |
strain background: wistar
strain: Spontaneously hypertensive rats (SHRs)
age: 8-10 week-old
gender: male
body weight (g): 280-330 g
treated with: HBMP 20 mg/kg/day for 28days
tissue: kidney
|
| Treatment protocol |
SHRs were randomly divided into three groups (n=10): control group, rats were orally administered with water (about 3 mL) for 4 weeks; low-dose group, rats were orally administered with HBMP (10 mg/kg/day, about 3 mL) for 4 weeks; high-dose group, rats were orally administered with HBMP (20 mg/kg/day, about 3 mL) for 4 weeks. The HBMP was orally administered at 9:00 every morning.
|
| Growth protocol |
SHRs were housed individually in steel cages with controlled room temperature (23 ± 1℃), humidity (55 ± 5%), and lighting (lights on from 06:00 to 18:00).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from each sample was extracted using TRK1001 (LC Sciences, Cat. TRK-1001, Total RNA Purification Kit)according to the manufacturer’s instructions. Total RNA was quantified by the NanoDrop ND-2000 (Thermo Scientific) and the RNA integrity was assessed using Agilent Bioanalyzer 2100 (Agilent Technologies).
|
| Label |
Cy3
|
| Label protocol |
The sample labeling, microarray hybridization and washing were performed based on the manufacturer’s standard protocols. Briefly, total RNA were transcribed to double strand cDNA, then synthesized into cRNA and labeled with Cyanine-3-CTP.
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| |
|
| Hybridization protocol |
The labeled cRNAs were hybridized onto the microarray according to the manufacturer’s standard protocols.
|
| Scan protocol |
After washing, arrays were scanned using GenePix 4000B scanner (Molecular Devices) according to the manufacturer’s protocol.
|
| Description |
High-dose_NormSignal
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| Data processing |
Feature Extraction software (GenePix® Pro 7, Axon Instruments, Inc.) was used to analyze array images to get raw data. To begin with, the raw data was normalized with the quantile algorithm. The probes that at least 1 out of all samples have flags in Detected were chosen for further data analysis. Differentially expressed genes were then identified through fold change as well as P value calculated with t-test. The threshold set for up- and down-regulated genes was a fold change>= 2.0 and a P value<= 0.05. expression pattern among samples.
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| |
|
| Submission date |
Sep 24, 2015 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Junli Feng |
| E-mail(s) |
[email protected]
|
| Organization name |
Zhejiang Gongshang University
|
| Street address |
Hangzhou, Jiaogong Road 149#
|
| City |
Hangzhou |
| ZIP/Postal code |
310012 |
| Country |
China |
| |
|
| Platform ID |
GPL14746 |
| Series (1) |
| GSE73387 |
Alteration of Gene Expression Profile in Spontaneously Hypertensive Rats Treated with Protein Hydrolysate of Blue Mussel (Mytilus edulis) by DNA Microarray Analysis |
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