|
| Status |
Public on Jun 23, 2015 |
| Title |
E11.5_dorsal cortex_litter2_mt2 |
| Sample type |
RNA |
| |
|
| Source name |
dorsal forebrain,E11.5
|
| Organism |
Mus musculus |
| Characteristics |
tissue: dorsal forebrain
dev stage: E11.5
genotype/variation: mutant
|
| Growth protocol |
Time-mated mice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Mothers were sacrificed and embryos isolated. Dorsal forebrain was dissected in ice-cold HBSS without Ca/Mg and snap-frozen before RNA isolation with RNAeasy kit with on-column DNase digestion (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
One-Color Microarray-Based Gene OneColor Microarray-Based Gene Expression Analysis, Agilent Technologies
|
| |
|
| Hybridization protocol |
Described in One-Color Microarray-Based Gene OneColor Microarray-Based Gene Expression Analysis, Agilent Technologies
|
| Scan protocol |
Raw data processing was performed using the Agilent Scan Control and the Agilent Feature Extraction Software Version 10
|
| Description |
Gene expression on E11.5 dorsal forebrain, directly dissected
|
| Data processing |
Spot intensities were loaded into R/Bioconductor and further processed with the limma package.
|
| |
|
| Submission date |
Jun 22, 2015 |
| Last update date |
Jun 23, 2015 |
| Contact name |
Lukas Sommer |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Zurich
|
| Department |
Anatomy
|
| Street address |
Winterthurerstr 190
|
| City |
Zurich |
| ZIP/Postal code |
8057 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL11202 |
| Series (1) |
| GSE70134 |
Wnt/b-catenin signaling regulates sequential fate decisions of murine cortical precursor cells |
|
