|
| Status |
Public on Jul 02, 2015 |
| Title |
Aristolochic acid-treated NRK-52E cells at 24h_biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
NRK-52E cells (DeLarco and Todaro, 1978) (ATCC, CRL-1571)
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Osborne-Mendel
cell line: NRK-52E renal proximal tubular cell line
agent: AA
time: 24h
|
| Treatment protocol |
NRK-52E cells were cultured to confluence on 6-well plates. Cells were then exposed to aristolochic acid dissolved in DMSO (0.1%) at the IC10 concentration at 72h (1.65μM) or DMSO only as control. After 6h, 24h and 72h the medium was removed and RNA was extracted from the cells. Three studies were conducted at each time point.
|
| Growth protocol |
NRK-52E cells (DeLarco and Todaro, 1978) (ATCC, CRL-1571) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) with 10% fetal calf serum, penicillin 100 I.U./ml, and streptomycin solution 100 µg/ml in a humidified 5% CO2 incubator at 370C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Rat Genome 230 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray scanner.
|
| Description |
Gene expression data from cells treated with aristolochic acids
AA-24h-R-2
|
| Data processing |
Signal condensation and normalization was performed using GC-RMA (Robust Multiarray Analysis algorithm) using GeneSpring GX11.
|
| |
|
| Submission date |
May 08, 2015 |
| Last update date |
Jul 02, 2015 |
| Contact name |
Kate Bloch |
| E-mail(s) |
[email protected]
|
| Organization name |
Manchester University
|
| Street address |
Alderley Park, Alderley Edge
|
| City |
Manchester |
| ZIP/Postal code |
SK10 4TG |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE68687 |
Expression data from NRK-52E cells treated with aristolochic acids for 6h, 24h and 72h |
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