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Series GSE68687 Query DataSets for GSE68687
Status Public on Jul 02, 2015
Title Expression data from NRK-52E cells treated with aristolochic acids for 6h, 24h and 72h
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary In this study we have examined the effect of sub-cytotoxic exposure to aristolochic acids (1.65µM) at 6h, 24h and 72h on the whole-genome expression profile in a rat proximal renal tubule cell line (NRK-52E).
We used microarrays to detail the mechanism of toxicity and possibly carcinogenicity of aristolochic acids in rat renal proximal cells.
 
Overall design NRK-52E cells were cultured to confluence on 6-well plates. Cells were then exposed to aristolochic acid dissolved in DMSO (0.1%) at the IC10 concentration at 72h (1.65 μM) or DMSO only as control. After 6h, 24h and 72h the medium was removed and RNA was extracted from the cells. Three studies were conducted at each time point.
 
Contributor(s) Bloch KM, Lock EA
Citation(s) 26484264
Submission date May 08, 2015
Last update date Jul 31, 2017
Contact name Kate Bloch
E-mail(s) [email protected]
Organization name Manchester University
Street address Alderley Park, Alderley Edge
City Manchester
ZIP/Postal code SK10 4TG
Country United Kingdom
 
Platforms (1)
GPL1355 [Rat230_2] Affymetrix Rat Genome 230 2.0 Array
Samples (18)
GSM1679033 Aristolochic acid-treated NRK-52E cells at 6h_biological rep1
GSM1679034 Aristolochic acid-treated NRK-52E cells at 6h_biological rep2
GSM1679035 Aristolochic acid-treated NRK-52E cells at 6h_biological rep3
Relations
BioProject PRJNA283393

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE68687_RAW.tar 46.6 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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