|
| Status |
Public on May 07, 2015 |
| Title |
Wild type liver, normal diet 2 |
| Sample type |
RNA |
| |
|
| Source name |
Wild type liver, normal diet
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
gender: Female
genotype/variation: Wild type
protocol: normal diet
tissue: liver
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from liver tissues using RNeasy Mini kit (Qiagen, Valencia, CA) following manufacture's protocol. Extracted RNA quality was checked by the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).
|
| Label |
Cy3
|
| Label protocol |
Cy3 labeled cRNA was prepared from 0.2 ug RNA using the One-Color Low Input Quick Amp Labeling kit (Agilent) according to the manufacturer's instructions, followed by RNeasy Mini column purification (QIAGEN, Valencia, CA). cRNA was quantified with the NanoDrop ND-1000 Spectrophotometer
|
| |
|
| Hybridization protocol |
The labelled cRNA was hybridized to the Agilent Whole Mouse Genome Microarray 4x44K v2 following manufacture's protocol.
|
| Scan protocol |
Arrays were scanned using Agilent G2565A Microarray Scanner System.
|
| Description |
MR162
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 9.1.3.1 (Agilent Technology) using default parameters.
The raw signal intensities were log2 transformed and normalized by 75 percentile shift using Gene spring GX software (Agilent Technology).
|
| |
|
| Submission date |
May 07, 2015 |
| Last update date |
May 07, 2015 |
| Contact name |
Yutaka Inagaki |
| E-mail(s) |
[email protected]
|
| Organization name |
Tokai University School of Medicine
|
| Department |
Regenerative Medicine
|
| Street address |
Shimokasuya 143
|
| City |
Isehara |
| State/province |
Kanagawa |
| ZIP/Postal code |
2591193 |
| Country |
Japan |
| |
|
| Platform ID |
GPL11202 |
| Series (1) |
| GSE68632 |
Gene expression Tet-mev-1 mouse fed either normal diet or high fat high sucrose diet compared with wild type mouse |
|
