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Sample GSM1664540 Query DataSets for GSM1664540
Status Public on Jun 02, 2015
Title Liver_PBS_5
Sample type RNA
 
Channel 1
Source name Liver_PBS
Organism Mus musculus
Characteristics tissue: Liver
treatment: PBS
genotype: db/db
Extracted molecule total RNA
Extraction protocol Total RNA was prepared by Qiagen RNeasy kit with on column DNase digestion.
Label Cy5
Label protocol Total RNA was converted to double-stranded cDNA and then into Cy-dye labeled cRNA using Agilent’s Quick Amp Labeling Kit. For all samples, 750 ng of the labeled cRNA was fragmented and hybridized against Cy3-labeled Universal mouse reference (Stratagene).
 
Channel 2
Source name Universal mouse reference (UMR) from Stratagene
Organism Mus musculus
Characteristics sample type: pooled mouse reference RNA
Extracted molecule total RNA
Extraction protocol Total RNA was prepared by Qiagen RNeasy kit with on column DNase digestion.
Label Cy3
Label protocol Total RNA was converted to double-stranded cDNA and then into Cy-dye labeled cRNA using Agilent’s Quick Amp Labeling Kit. For all samples, 750 ng of the labeled cRNA was fragmented and hybridized against Cy3-labeled Universal mouse reference (Stratagene).
 
 
Hybridization protocol Agilent In Situ Hybridization Kit Plus was used where Cy-dye labeled control and test samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, the arrays were washed twice, dried and then scanned.
Scan protocol Scanned on an Agilent scanner, images were processed using Agilent Feature Extraction software version 11.
Data processing Agilent Feature Extraction Software version 11 was used for background subtraction and LOWESS normalization.
 
Submission date Apr 21, 2015
Last update date Jun 02, 2015
Contact name Matthew Brauer
E-mail(s) [email protected]
Organization name Genentech, Inc.
Department Bioinformatics
Street address 1 DNA Way
City South San Francisco
State/province California
ZIP/Postal code 94080
Country USA
 
Platform ID GPL11202
Series (2)
GSE68142 Anti-GCGR antibody treatment in db/db mice
GSE68145 Glucagon Couples Hepatic Amino Acid Catabolism to mTOR-dependent Regulation of α-cell Mass

Data table header descriptions
ID_REF
VALUE Normalized log2 ratio (Cy5/Cy3) representing test/reference

Data table
ID_REF VALUE
A_55_P1989846 -0.521426296
A_55_P1991598 -0.26050085
A_55_P2022211 -0.943468082
A_55_P1980764 -0.058734334
A_55_P1964375 -0.508493957
A_51_P128876 0.79890311
A_55_P2121042 -0.075001827
A_52_P219230 -0.282737037
A_51_P207591 -2.590026515
A_55_P2131920 -4.194859202
A_55_P2404223 -0.257656332
A_55_P2101944 1.536246734
A_52_P358860 1.677167192
A_51_P119031 0.217071387
A_51_P309854 4.84139194
A_51_P343900 -1.024012476
A_51_P234359 0.050319787
A_51_P487813 -4.09793209
A_52_P613977 0.167863018
A_55_P1957209 -0.2851185

Total number of rows: 39429

Table truncated, full table size 1000 Kbytes.




Supplementary file Size Download File type/resource
GSM1664540_Agilent_252665516032_S001_GE2_1105_Oct12_1_2.txt.gz 4.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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