|
| Status |
Public on Apr 14, 2015 |
| Title |
LD-3 |
| Sample type |
RNA |
| |
|
| Source name |
serum form LD SCLC patient
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
age: 56y
subject status: small cell lung cancer (SCLC) patient
Stage: limited-stage
tissue: patient's serum
|
| Treatment protocol |
All serum was collected before any treatment
|
| Growth protocol |
Patients' blood were collected and seperated in 4hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
200ng of total RNA were used to fellow expriment follow miRNA Complete Labeling and Hyb Kit (Agilent) protocol.
|
| |
|
| Hybridization protocol |
Follow miRNA Complete Labeling and Hyb Kit (Agilent) protocol.
|
| Scan protocol |
Scanned on an Agilent G2565CA scanner.
|
| Description |
miRNAs expression before treatment
050
|
| Data processing |
Agilent Feature Extraction(v10.7)was used for background subtraction and Agilent GeneSpring was used to normalization.
|
| |
|
| Submission date |
Apr 13, 2015 |
| Last update date |
Apr 14, 2015 |
| Contact name |
Rui Zhou |
| E-mail(s) |
[email protected]
|
| Phone |
+8613971523840
|
| Organization name |
Wuhan Union Hospital
|
| Department |
Cancer Center
|
| Street address |
湖北省武汉市江汉区唐家墩156号
|
| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430023 |
| Country |
China |
| |
|
| Platform ID |
GPL16543 |
| Series (1) |
| GSE67804 |
miRNAs related to metastasis of Small Cell Lung Cancer |
|
