|
| Status |
Public on Jan 23, 2018 |
| Title |
4T1-nSMase2-OE-exosome |
| Sample type |
RNA |
| |
|
| Source name |
4T1-nSMase2-OE cells
|
| Organism |
Mus musculus |
| Characteristics |
source of rna: exosome
cell line: 4T1
genotype: nSMase2-OE
|
| Treatment protocol |
These cells were genetically modified by shRNA or expression vector against nSMase2
|
| Growth protocol |
Culture in RPMI1640 medium with 10% FCS and Antibiotic-Antimycotic
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Isolation of exosomal and cellular RNAs was performed using the miRNeasy Mini Kit according to the manufacture instruction.
|
| Label |
Cy3
|
| Label protocol |
100 ng of each RNA sample was labeled with the miRNA Complete Labeling Reagent and Hybridization kit (Agilent Technologies) according to the manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
Labeled RNA was hybridized to the mouse miRNA microarray, Release 18.0 (G4872A#38112 Agilent Technologies) with the miRNA Complete Labeling Reagent and Hybridization kit (Agilent Technologies) according to the manufacturer’s instructions.
|
| Scan protocol |
Microarray slides were scanned in an Agilent Technologies G2505C Microarray Scanner at 3 micron resolution.
|
| Description |
Exosomal total RNA from 4T1 cells whose expression of nSMase2 was over-expressed.
|
| Data processing |
Images were quantified using Agilent Feature Extraction software version 10.7.3.1 (Agilent) using default parameters (Grid:038112_D_F_20120106) to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
|
| |
|
| Submission date |
Jan 23, 2015 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Nobuyoshi Kosaka |
| E-mail(s) |
[email protected]
|
| Organization name |
National Cancer Center
|
| Department |
Research of Molecular Functions and Targets
|
| Lab |
Division of Molecular and Cellular Medicine
|
| Street address |
5-1-1, Tsukiji, Chuo-ku
|
| City |
Tokyo |
| ZIP/Postal code |
104-0045 |
| Country |
Japan |
| |
|
| Platform ID |
GPL16199 |
| Series (1) |
| GSE65260 |
Neutral sphingomyelinase 2 (nSMase2)-dependent exosomal transfer of angiogenic microRNAs regulate cancer cell metastasis |
|
