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Sample GSM158509 Query DataSets for GSM158509
Status Public on Mar 01, 2007
Title 1000cGY replicate 5
Sample type RNA
 
Channel 1
Source name 1000cGY replicate 5
Organism Mus musculus
Characteristics MNCs were plelleted and total RNA was isolated via RNAeasy minispin column
Biomaterial provider John Chute Lab
Treatment protocol 1000cGy
Growth protocol no growth protocol
Extracted molecule total RNA
Extraction protocol Briefly, 5 x 106 MNCs were pelleted and total RNA was isolated using the RNAeasy minispin column
Label cy13
Label protocol http://microarray.genome.duke.edu/spotted-arrays/protocols/Ambion_AmpII_protocol
 
Channel 2
Source name Stratagene Universal Mouse reference
Organism Mus musculus
Characteristics http://www.stratagene.com/manuals/740100.pdf
Biomaterial provider Stratagene
Treatment protocol No treatment
Growth protocol no growth protocol
Extracted molecule total RNA
Extraction protocol Briefly, 5 x 106 MNCs were pelleted and total RNA was isolated using the RNAeasy minispin column
Label cy11
Label protocol http://microarray.genome.duke.edu/spotted-arrays/protocols/Ambion_AmpII_protocol
 
 
Hybridization protocol http://mgm.duke.edu/genome/dna_micro/core/protocols/SOP_hyb.doc
Scan protocol http://microarray.genome.duke.edu/spotted-arrays/protocols
Description http://www.stratagene.com/manuals/740100.pdf
Data processing Data was analysed with GeneSpring 7 data software package (Silicon Genetics). Each data set was filtered based on 1.5X background, thus there are missing data ( ) and normalised using Lowess intensity-dependent normalisation.
 
Submission date Jan 25, 2007
Last update date Jan 25, 2007
Contact name Holly Kloos Dressman
E-mail(s) [email protected]
Organization name Duke University Medical Center
Department Institute for Genome Sciences and Policy
Street address 101 Science Drive
City Durham
State/province NC
ZIP/Postal code 27708
Country USA
 
Platform ID GPL4783
Series (2)
GSE6873 Gene expression signatures that predict radiation exposure (mouse)
GSE6874 Gene expression signatures that predict radiation exposure

Data table header descriptions
ID_REF
VALUE normalized/filtered log2ratio data (cy5/cy3)
PRE_VALUE normalized/filtered ratio data (cy5/cy3)

Data table
ID_REF VALUE PRE_VALUE
M200000001 -0.9406 0.521
M200000002 -1.4501 0.366
M200000004 -3.3437 0.0985
M200000006 0.3606 1.284
M200000009 -1.8314 0.281
M200000010 -3.5146 0.0875
M200000011 0.7181 1.645
M200000012 -2.2379 0.212
M200000016 0.0072 1.005
M200000017 -1.7563 0.296
M200000020 2.0507 4.143
M200000021 2.7613 6.78
M200000023 -0.4074 0.754
M200000025 -0.5864 0.666
M200000028 1.3254 2.506
M200000030 -4.0783 0.0592
M200000031 0.6871 1.61
M200000032 -0.8863 0.541
M200000033 -1.8625 0.275
M200000037 4.0976 17.12

Total number of rows: 16722

Table truncated, full table size 399 Kbytes.




Supplementary file Size Download File type/resource
GSM158509.gpr.gz 2.9 Mb (ftp)(http) GPR

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