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Sample GSM158491 Query DataSets for GSM158491
Status Public on Mar 01, 2007
Title 50cGy replicate 1
Sample type RNA
 
Channel 1
Source name 50cGy replicate 1
Organism Mus musculus
Characteristics MNCs were plelleted and total RNA was isolated via RNAeasy minispin column
Biomaterial provider John Chute Lab
Treatment protocol 50cGy
Growth protocol no growth protocol
Extracted molecule total RNA
Extraction protocol Briefly, 5 x 106 MNCs were pelleted and total RNA was isolated using the RNAeasy minispin column
Label cy5
Label protocol http://microarray.genome.duke.edu/spotted-arrays/protocols/Ambion_AmpII_protocol
 
Channel 2
Source name Stratagene Universal Mouse reference
Organism Mus musculus
Characteristics http://www.stratagene.com/manuals/740100.pdf
Biomaterial provider Stratagene
Treatment protocol No treatment
Growth protocol no growth protocol
Extracted molecule total RNA
Extraction protocol Briefly, 5 x 106 MNCs were pelleted and total RNA was isolated using the RNAeasy minispin column
Label cy3
Label protocol http://microarray.genome.duke.edu/spotted-arrays/protocols/Ambion_AmpII_protocol
 
 
Hybridization protocol http://mgm.duke.edu/genome/dna_micro/core/protocols/SOP_hyb.doc
Scan protocol http://microarray.genome.duke.edu/spotted-arrays/protocols
Description http://www.stratagene.com/manuals/740100.pdf
Data processing Data was analysed with GeneSpring 7 data software package (Silicon Genetics). Each data set was filtered based on 1.5X background, thus there are missing data ( ) and normalised using Lowess intensity-dependent normalisation.
 
Submission date Jan 25, 2007
Last update date Sep 17, 2009
Contact name Holly Kloos Dressman
E-mail(s) [email protected]
Organization name Duke University Medical Center
Department Institute for Genome Sciences and Policy
Street address 101 Science Drive
City Durham
State/province NC
ZIP/Postal code 27708
Country USA
 
Platform ID GPL4783
Series (2)
GSE6873 Gene expression signatures that predict radiation exposure (mouse)
GSE6874 Gene expression signatures that predict radiation exposure

Data table header descriptions
ID_REF
VALUE normalized/filtered log2ratio data (cy5/cy3)
PRE_VALUE normalized/filtered ratio data (cy5/cy3)

Data table
ID_REF VALUE PRE_VALUE
M200000001 -2.9105 0.133
M200000002 -2.8997 0.134
M200000004 -2.8678 0.137
M200000006 -0.5951 0.662
M200000009 -1.3147 0.402
M200000010 -3.3688 0.0968
M200000011 1.1104 2.159
M200000012 -1.7179 0.304
M200000016 -0.8651 0.549
M200000017 -0.7661 0.588
M200000020 3.3434 10.15
M200000021 1.0649 2.092
M200000023 -0.5249 0.695
M200000025 0.4016 1.321
M200000028 0.6022 1.518
M200000030 -3.5581 0.0849
M200000031 0.5636 1.478
M200000032 -0.0985 0.934
M200000033 -0.6781 0.625
M200000037 2.6512 6.282

Total number of rows: 16722

Table truncated, full table size 398 Kbytes.




Supplementary file Size Download File type/resource
GSM158491.gpr.gz 2.8 Mb (ftp)(http) GPR

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