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Sample GSM158485 Query DataSets for GSM158485
Status Public on Mar 01, 2007
Title Normal replicate 2
Sample type RNA
 
Channel 1
Source name Normal replicate 2
Organism Mus musculus
Characteristics MNCs were plelleted and total RNA was isolated via RNAeasy minispin column
Biomaterial provider John Chute Lab
Treatment protocol no treatment
Growth protocol no growth protocol
Extracted molecule total RNA
Extraction protocol Briefly, 5 x 106 MNCs were pelleted and total RNA was isolated using the RNAeasy minispin column
Label cy5
Label protocol http://microarray.genome.duke.edu/spotted-arrays/protocols/Ambion_AmpII_protocol
 
Channel 2
Source name Stratagene Universal Mouse reference
Organism Mus musculus
Characteristics http://www.stratagene.com/manuals/740100.pdf
Biomaterial provider Stratagene
Treatment protocol No treatment
Growth protocol no growth protocol
Extracted molecule total RNA
Extraction protocol Briefly, 5 x 106 MNCs were pelleted and total RNA was isolated using the RNAeasy minispin column
Label cy3
Label protocol http://microarray.genome.duke.edu/spotted-arrays/protocols/Ambion_AmpII_protocol
 
 
Hybridization protocol http://mgm.duke.edu/genome/dna_micro/core/protocols/SOP_hyb.doc
Scan protocol http://microarray.genome.duke.edu/spotted-arrays/protocols
Description http://www.stratagene.com/manuals/740100.pdf
Data processing Data was analysed with GeneSpring 7 data software package (Silicon Genetics). Each data set was filtered based on 1.5X background, thus there are missing data ( ) and normalised using Lowess intensity-dependent normalisation.
 
Submission date Jan 25, 2007
Last update date Sep 17, 2009
Contact name Holly Kloos Dressman
E-mail(s) [email protected]
Organization name Duke University Medical Center
Department Institute for Genome Sciences and Policy
Street address 101 Science Drive
City Durham
State/province NC
ZIP/Postal code 27708
Country USA
 
Platform ID GPL4783
Series (2)
GSE6873 Gene expression signatures that predict radiation exposure (mouse)
GSE6874 Gene expression signatures that predict radiation exposure

Data table header descriptions
ID_REF
VALUE normalized/filtered log2ratio data (cy5/cy3)
PRE_VALUE normalized/filtered ratio data (cy5/cy3)

Data table
ID_REF VALUE PRE_VALUE
M200000001 -2.0058 0.249
M200000002 -1.9602 0.257
M200000004 -2.7661 0.147
M200000006 -0.2076 0.866
M200000009 -1.1172 0.461
M200000010 -3.2243 0.107
M200000011 1.0496 2.07
M200000012 -1.7959 0.288
M200000016 -0.9214 0.528
M200000017 -1.1109 0.463
M200000020 3.1969 9.17
M200000021 1.2154 2.322
M200000023 -0.9324 0.524
M200000025 0.0314 1.022
M200000028 -0.0589 0.96
M200000030 -6.6439 0.01
M200000031 0.7338 1.663
M200000032 -0.5146 0.7
M200000033 -0.6394 0.642
M200000037 4.4456 21.79

Total number of rows: 16722

Table truncated, full table size 397 Kbytes.




Supplementary file Size Download File type/resource
GSM158485.gpr.gz 2.9 Mb (ftp)(http) GPR

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