|
| Status |
Public on Aug 03, 2016 |
| Title |
ileum stressed rep1 |
| Sample type |
RNA |
| |
|
| Source name |
teminal ileum of stressed mouse
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6JJmsSlc
treatment: stressed
tissue: teminal ileum
|
| Treatment protocol |
The sCSDS paradigm was performed in the afternoon (14:00 pm-16:00 pm). The duration of physical contacts was set at 5 min after the first attack bite at Day 1, and then was reduced 0.5 min per day from Day 2 to Day 10. Thus, 0.5 min of physical contact was carried out at Day 10.
|
| Growth protocol |
Male C57BL/6JJmsSlc (B6) mice (7 weeks of age; 21-23 g) for intruders and male Slc:ICR (ICR) mice (retired, older than 5 months of age) for residents (purchased from SLC Japan, Shizuoka, Japan) were used. First, these mice were housed individually in single cages (cage size 143 mm × 293 mm × 148 mm; Charles River Laboratories Japan, Kanagawa, Japan) with wood-shaving bedding and were habituated to their environments for a week. AIN-93G diet (Oriental Yeast, Tokyo, Japan) was fed to B6 mice, and standard laboratory diet (MF diet; Oriental Yeast) was fed to ICR mice. Food and water were available ad libitum. Animals were maintained at constant temperature (23 ± 1°C) and humidity (61 ± 1%) under a 12 h light-dark cycle (light on 8:00 am).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After 3 hr fasting, the lower small intestines were taken and immediately kept in RNAlater (Qiagen). RNA was prepared by RNeasy kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
cRNA was prepared using the Affymetrix GeneChip 3’IVT Epess kit, using 100 ng total RNA as starting material.
|
| |
|
| Hybridization protocol |
Fragmented cRNA was hybridised to mouse 430 2.0 arrays (Affymetrix) during 16 h at 45°C.
|
| Scan protocol |
After hybridization, the gene chips were washed and stained using a GeneChip Fluidics Station 450 (Affymetrix) and then scanned (GeneChip Scanner; Affymetrix) with the GeneChip Operation Software Ver. 1.4 (Affymetrix).
|
| Description |
Gene expression data from ileum of mouse suffered from subchronic and mild social defeat stress
|
| Data processing |
The microarray data were analyzed using R. Affymetrix CEL files were quantified by the RMA summarization.
|
| |
|
| Submission date |
Dec 09, 2014 |
| Last update date |
Aug 03, 2016 |
| Contact name |
Chise Suzuki |
| Organization name |
NARO Institute of Livestock and Grassland Science
|
| Department |
Animal Products Res. Division
|
| Lab |
Functional Biomolecules Res. Group
|
| Street address |
2 Ikenodai
|
| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-0901 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE64004 |
Expression data from ileum of mice suffered from subchronic and mild social defeat stress |
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