|
| Status |
Public on Nov 20, 2014 |
| Title |
Damaged interstitium 1 |
| Sample type |
RNA |
| |
|
| Source name |
Laser capture microdissection
|
| Organism |
Mus musculus |
| Characteristics |
tissue: distal lung
state: damage from influenza infected mice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Pico pure RNA isolation kit from Arcturus.
|
| Label |
biotin
|
| Label protocol |
RNA was amplified and labelled using WT Pico RNA Amplification System, WT-Ovation Exon Module and Encore Biotin Module (NuGEN Technologies)
|
| |
|
| Hybridization protocol |
Hybridization was done using standard Affymetrix protocols
|
| Scan protocol |
GeneChip® Scanner 3000,Affymetrix
|
| Description |
RMA expression value derived from Expression Console software; core-exon analysis
|
| Data processing |
The intensity values were Log2-transformed and imported into the Partek Genomics Suite 6.6 (Partek Inc, USA). Exons were summarized to genes and 1-way ANOVA was performed to identify differentially expressed genes. P values and fold-change were calculated for each analysis.
|
| |
|
| Submission date |
Sep 03, 2014 |
| Last update date |
Nov 20, 2014 |
| Contact name |
Frank McKeon |
| E-mail(s) |
[email protected]
|
| Phone |
8608372486
|
| Organization name |
University of Houston
|
| Department |
Biology and Biochemistry
|
| Street address |
3455 Cullen Blvd
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77004 |
| Country |
USA |
| |
|
| Platform ID |
GPL6193 |
| Series (2) |
| GSE60849 |
p63+/Krt5+ Distal Airway Stem Cells are Essential for Lung Regeneration |
| GSE61091 |
p63+/Krt5+ Distal Airway Stem Cells are Essential for Lung Regeneration (tracheal epithelium and alveoli) |
|
