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| Status |
Public on Nov 20, 2014 |
| Title |
p63+/Krt5+ Distal Airway Stem Cells are Essential for Lung Regeneration (tracheal epithelium and alveoli) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The possibility of lung regeneration has been long discounted due to the irreversible nature of chronic lung diseases. However, patients who sustain massive loss of lung tissue during acute infections often recover full pulmonary function. Correspondingly, we previously demonstrated lung regeneration in mice following H1N1 influenza virus infection and implicated p63+Krt5+ distal airway stem cells, or DASCp63/Krt5, in this process. We show here that rare, preexisting DASCp63/K5 undergo a proliferative expansion in response to influenza and lineage-trace to nascent alveoli assembled at sites of interstitial inflammation. We also show that the ablation of DASCp63/Krt5 in vivo prevents the regeneration of lung tissue following influenza leading to pre-fibrotic lesions and deficient oxygen exchange. Finally, we demonstrate that exogenously cloned and propagated DASCp63/Krt5 readily contribute to lung regeneration following transplantation. These data suggest that DASCp63/K5 are required for lung regeneration and may have therapeutic utility in acute and chronic lung diseases.
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| Overall design |
Tracheal epithelium and alveoli of healthy mice were laser capture microdissected for microarray analysis. Damaged lung interstitium (CD45+ region) of influenza infected (75pfu H1N1, 15dpi) mice were also dissected. Duplicates were included for each sample. We used the Affymetrix Mouse Exon 1.0 ST platform
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| Contributor(s) |
Zuo W, Zhang T, Wu DZ, Guan S, Liew AA, Yamamoto Y, Lim TS, Vicent M, Lessard M, Crum CP, Xian W, McKeon F |
| Citation(s) |
25383540 |
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| Submission date |
Sep 03, 2014 |
| Last update date |
Mar 03, 2017 |
| Contact name |
Frank McKeon |
| E-mail(s) |
[email protected]
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| Phone |
8608372486
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| Organization name |
University of Houston
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| Department |
Biology and Biochemistry
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| Street address |
3455 Cullen Blvd
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| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77004 |
| Country |
USA |
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| Platforms (1) |
| GPL6193 |
[MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [probe set (exon) version] |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE60849 |
p63+/Krt5+ Distal Airway Stem Cells are Essential for Lung Regeneration |
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| Relations |
| BioProject |
PRJNA260211 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE61091_RAW.tar |
131.7 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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