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Sample GSM1465289 Query DataSets for GSM1465289
Status Public on Aug 06, 2014
Title 18 1500 ppm of hexanal was exposed in rats(T3 group)
Sample type RNA
 
Source name Lung, hexanal, 1500 ppm
Organism Rattus norvegicus
Characteristics tissue: lung
gender: male
treatment: 1500 ppm hexanal
Treatment protocol Hexanal was inhaled for 5 weeks in Fischer-344 rats(approximately 7-8 weeks old). Atmospheres of hexanal aerosol were generated at target concentrations of 0(control), 600, 1000, 1500 mg/m3 in air. Rats were exposed to the target concentrations for 4h/day, 5days/week.
Growth protocol Fischer-344 rats of both sexes were obtained from at the age of 7-8 weeks from OrientBio, Inc(Korea). The animals were quarantined and acclimated for 5days before the start of experiment. The animals were housed in stainless-steel wire hanging cages(255W×465L×200H ㎜).The environment in the exposure chamber was maintained constant at a temperature of 22±3 ℃ and a relative humadity of 30-70%. All rats were given sterilized commercial pellet diet ( PMI Nutrition International, USA) and sterilized water.
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy3
Label protocol Amplified and labeled cRNA was purified on cRNA Cleanup Module (Agilent Technology) according to the manufacturer’s protocol. Labeled cRNA target was quantified using ND-1000 spectrophotometer (NanoDrop Technologies, Inc., Wilmington, DE).
 
Hybridization protocol After checking labeling efficiency, fragmentation of cRNA was performed by adding 10X blocking agent and 25X fragmentation buffer and incubating at 60oC for 30 min. The fragmented cRNA was resuspended with 2X hybridization buffer and directly pipetted onto assembled Agilent’s Canine Oligo Microarray (44K). The arrays hybridized at 65oC for 17 hours using Agilent Hybridization oven (Agilent Technology, USA). The hybridized microarrays were washed as the manufacturer’s washing protocol (Agilent Technology, USA).
Scan protocol The hybridized images were scanned using Agilent’s DNA microarray scanner and quantified with Feature Extraction Software (Agilent Technology, Palo Alto, CA).
Description Gene expression after exposure to 1500ppm hexanal
Data processing All data normalization and further analysis were performed using GeneSpringGX 7.3 (Agilent Technology, USA).
 
Submission date Aug 05, 2014
Last update date Aug 08, 2014
Contact name Yoon Cho
E-mail(s) [email protected]
Phone 8201094948085
Organization name KIST
Street address hwarang-ro 14
City Seoul
ZIP/Postal code 02792
Country South Korea
 
Platform ID GPL14746
Series (2)
GSE60118 Inhalation exposure of hexanal in rats (mRNA)
GSE60205 Inhalation exposure of hexanal in rats

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
A_42_P453055 16542.33
A_42_P453171 6470.0845
A_42_P453894 5125.376
A_42_P453935 38157.363
A_42_P453959 42.97647
A_42_P453976 176.41061
A_42_P454206 540.2913
A_42_P454301 9249.427
A_42_P454311 1815.026
A_42_P454378 67.57777
A_42_P455785 6221.8906
A_42_P455802 4464.898
A_42_P456080 32.490566
A_42_P456155 32625.77
A_42_P456701 2070.201
A_42_P457003 15541.757
A_42_P457692 5961.089
A_42_P457773 8031.629
A_42_P457783 173.926
A_42_P457895 624.54047

Total number of rows: 30367

Table truncated, full table size 649 Kbytes.




Supplementary file Size Download File type/resource
GSM1465289__18.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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