|
| Status |
Public on Nov 21, 2014 |
| Title |
100_spiked_MCF7_a |
| Sample type |
RNA |
| |
|
| Source name |
breast cancer
|
| Organism |
Homo sapiens |
| Characteristics |
experiment: 1
sample type: spiked cells
cell line: MCF7
cell number 5ml: 100
donor: d1
replicate: a
tissue: blood
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction was performed according to the manufacturer’s instructions and RNA was eluted in 5 or 10 ul of RNase-free water (Ambion), depending on the experimental setting.
|
| Label |
Cy3
|
| Label protocol |
Flourescently labeled cRNA were prepared according to the standard Illumina protocol.
|
| |
|
| Hybridization protocol |
cRNA were hybridazed onto Illumina HumanHT-12 WG-DASL V4.0 R2 expression beadchip according to the standard Illumina protocol.
|
| Scan protocol |
Illumina BeadArray Reader was used for scanning the arrays and Illumina BeadScan was used for image acquisition and recovery of primary data.
|
| Description |
healthy donor1 blood spiked with 100 MCF7 cells
|
| Data processing |
Raw data were generated using the Illumina BeadStudio 3.8 software and analyzed with R/Bioconductor. For each gene, only the probe with higher interquartile range was selected.
According with the characteristics of the study, data normalization was not applicable
|
| |
|
| Submission date |
Feb 28, 2014 |
| Last update date |
Nov 21, 2014 |
| Contact name |
Maurizio Callari |
| E-mail(s) |
[email protected]
|
| Organization name |
Fondazione IRCCS Istituto Nazionale dei Tumori
|
| Street address |
Via Amadeo, 42
|
| City |
Milan |
| ZIP/Postal code |
20133 |
| Country |
Italy |
| |
|
| Platform ID |
GPL14951 |
| Series (1) |
| GSE55470 |
Gene expression profiling of circulating tumor cells in breast cancer |
|
