|
| Status |
Public on Aug 22, 2014 |
| Title |
47_Lund_AS_sample Secondary |
| Sample type |
RNA |
| |
|
| Source name |
FFPE tumor tissue
|
| Organism |
Homo sapiens |
| Characteristics |
Sex: f
year of birth: 1920
year of breast cancer: 1992
year of angiosarcoma: 2001
replicate: Biol repl H1
previous radiotherapy to tumor area: Yes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Non-necrotic tumor areas were macro-dissected from FFPE blocks and RNA extraction was performed from three 10-µm sections using the High Pure RNA Paraffin Kit (Roche, Castle Hill, Australia) according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Standard Illumina labeling protocol (Ligated products were PCR-amplified and labeled with a universal Cy3-coupled primer, after which single-stranded labeled products were precipitated and then hybridized to WG gene expression BeadChips as previously described (Kuhn et al. 2004).)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina iScan N240 scanning protocol.
|
| Description |
Secondary
|
| Data processing |
Gene expression data were uploaded and processed in the GenomeStudio software (Illumina, San Diego, CA). Data were normalized using background correction and the cubic spline method.
|
| |
|
| Submission date |
Nov 22, 2013 |
| Last update date |
Aug 22, 2014 |
| Contact name |
Emelie Styring |
| E-mail(s) |
[email protected]
|
| Organization name |
Lund University
|
| Department |
Dept of Orthopedics
|
| Street address |
Skanes University Hospital
|
| City |
Lund |
| ZIP/Postal code |
22185 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL14951 |
| Series (1) |
| GSE52664 |
Genome-wide analysis of primary and secondary angiosarcomas using RNA extracted from formalin-fixed parafinembedded samples. |
|
