|
| Status |
Public on Feb 05, 2007 |
| Title |
mouse hippocampus, cox-2 knockout, rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Hippocampus COX-2 KO
|
| Organism |
Mus musculus |
| Characteristics |
Genotype: COX-2 KO
Age: 12 weeks of age
|
| Treatment protocol |
Mice were euthanized with pentobarbital overdose, decapitated and brain was frozen on dry ice after dissection into Cerebral cortex and hippocampus. Samples were stored at -80C until usage
|
| Growth protocol |
12/12 light cycle, ad libitum food
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed using the Qiagen Rneasy Lipid tissue extraction kit according to the manufacturer's instructions.
|
| Label |
33P
|
| Label protocol |
P33 labelled cDNA was prepared as described using the protocol found at http://www.grc.nia.nih.gov/branches/rrb/dna/dna.htm
|
| |
|
| Hybridization protocol |
Samples were hybridized to custom nylon cDNA array. Details of hybridization protocol can be found at http://www.grc.nia.nih.gov/branches/rrb/dna/dna.htm
|
| Scan protocol |
Arrays apposed to phosphoimager plate and scanned according to protocol found at http://www.grc.nia.nih.gov/branches/rrb/dna/dna.htm
|
| Description |
mouse hippocampus from COX-2 knockout
|
| Data processing |
The data were analyzed as described at http://www.grc.nia.nih.gov/branches/rrb/dna/dna.htm. Data are presented as z-ratios
|
| |
|
| Submission date |
Jul 18, 2006 |
| Last update date |
Jan 31, 2007 |
| Contact name |
Christopher Dennis Toscano |
| E-mail(s) |
[email protected]
|
| Phone |
301-594-3890
|
| Fax |
301-402-0074
|
| Organization name |
National Institutes of Health
|
| Department |
National Institute on Aging
|
| Lab |
Brain Physiology and Metabolism Section
|
| Street address |
9 Memorial Drive Room 1S126
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL4006 |
| Series (1) |
| GSE5342 |
Expression data from Cerebral Cortex or Hippocampus of COX-1 or COX-2 null mice |
|
