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| Status |
Public on Feb 05, 2007 |
| Title |
Expression data from Cerebral Cortex or Hippocampus of COX-1 or COX-2 null mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To further understand the physiological role of cyclooxygenase (COX), the critical enzyme involved in the production of eicosanoids from arachidonic acid, we performed microarray analysis of gene expression in the cerebral cortex and hippocampus of mice deficient in COX-1 (COX-1-/-) or COX-2 (COX-2-/-). Expression of lipid (ATP citrate lyase, acetyl-CoA acetyltransferase, hydroxyacyl-CoA dehydrogenase) and homocysteine (methionine adenosyltransferase, S-adenosylhomocysteine hydrolase) metabolism genes was increased in the cerebral cortex of COX-2 -/- mice. Further, expression of GABAergic neurotransmission genes (GABA transporter 3, GABA-A receptor subunit ?1) was altered in the cerebral cortex and hippocampus of COX-2 -/- mice. A COX isoform specific effect was observed in the expression of Janus Kinase (JAK) 1 and 2. COX-1 -/- mice exhibited an increase in JAK1 expression while COX-2 -/- mice exhibited a decrease in JAK2 expression, an observation consistent with a previously demonstrated COX isoform specific effect on the expression of NF-kB. In summary, this study demonstrates the wide ranging effects of genetic deletion of COX isoforms in the mouse brain and suggests that inhibition of COX activity may alter the profile of gene expression in specific areas of the brain.
We used nylon cDNA microarrays to detail the global programme of gene expression in the Cerebral Cortex and Hippocampus of COX-1 and COX-2 null (knockout) mice
Keywords: brain, cerebral cortex, hippocampus, COX-1 WT, COX-1 KO, COX-2 WT, COX-2 KO and COX-2 heterozygous
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| Overall design |
Mice were sacrificed at 12 weeks of age and the brain was dissected into cerebral cortex and hippocampus. We analyzed the overall gene expression from the cortex and hippocampus of COX-1 +/+, COX-1 -/-, COX-2 +/+, COX-2 -/-, and COX-2 +/-
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| Contributor(s) |
Toscano CD, Bosetti F, Prabhu VV, Becker KG, Langenbach R |
| Citation(s) |
17266762 |
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| Submission date |
Jul 18, 2006 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Christopher Dennis Toscano |
| E-mail(s) |
[email protected]
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| Phone |
301-594-3890
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| Fax |
301-402-0074
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| Organization name |
National Institutes of Health
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| Department |
National Institute on Aging
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| Lab |
Brain Physiology and Metabolism Section
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| Street address |
9 Memorial Drive Room 1S126
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
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| Samples (30)
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| GSM121421 |
mouse cerebral cortex, cox-1 WT, rep1 |
| GSM121422 |
mouse cerebral cortex, cox-1 WT, rep2 |
| GSM121423 |
mouse cerebral cortex, cox-1 WT, rep3 |
| GSM121424 |
mouse cerebral cortex, cox-1 knockout, rep1 |
| GSM121425 |
mouse cerebral cortex, cox-1 knockout, rep2 |
| GSM121426 |
mouse cerebral cortex, cox-1 knockout, rep3 |
| GSM121427 |
mouse cerebral cortex, cox-2 WT, rep1 |
| GSM121428 |
mouse cerebral cortex, cox-2 WT, rep2 |
| GSM121429 |
mouse cerebral cortex, cox-2 WT, rep3 |
| GSM121430 |
mouse cerebral cortex, cox-2 knockout, rep1 |
| GSM121431 |
mouse cerebral cortex, cox-2 knockout, rep2 |
| GSM121432 |
mouse cerebral cortex, cox-2 knockout, rep3 |
| GSM121433 |
mouse cerebral cortex, cox-2 heterozygous, rep1 |
| GSM121434 |
mouse cerebral cortex, cox-2 heterozygous, rep2 |
| GSM121435 |
mouse cerebral cortex, cox-2 heterozygous, rep3 |
| GSM121436 |
mouse hippocampus, cox-1 WT, rep1 |
| GSM121437 |
mouse hippocampus, cox-1 WT, rep2 |
| GSM121438 |
mouse hippocampus, cox-1 WT, rep3 |
| GSM121439 |
mouse hippocampus, cox-1 knockout, rep1 |
| GSM121440 |
mouse hippocampus, cox-1 knockout, rep2 |
| GSM121441 |
mouse hippocampus, cox-1 knockout, rep3 |
| GSM121442 |
mouse hippocampus, cox-2 WT, rep1 |
| GSM121443 |
mouse hippocampus, cox-2 WT, rep2 |
| GSM121444 |
mouse hippocampus, cox-2 WT, rep3 |
| GSM121445 |
mouse hippocampus, cox-2 knockout, rep1 |
| GSM121446 |
mouse hippocampus, cox-2 knockout, rep2 |
| GSM121447 |
mouse hippocampus, cox-2 knockout, rep3 |
| GSM121448 |
mouse hippocampus, cox-2 heterozygous, rep1 |
| GSM121449 |
mouse hippocampus, cox-2 heterozygous, rep2 |
| GSM121450 |
mouse hippocampus, cox-2 heterozygous, rep3 |
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| Relations |
| BioProject |
PRJNA96197 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE5342_RAW.tar |
375.9 Mb |
(http)(custom) |
TAR (of GEL) |
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