|
| Status |
Public on Nov 06, 2014 |
| Title |
adult oligodendrocytes_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
adult oligodendrocytes
|
| Organism |
Mus musculus |
| Characteristics |
strain: CR57BL/6
cell type: Primary FAC-sorted adult OLs
treatment: Control
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were obtained from FAC-sorted neonatal or 2-month-old progenitors of oligodendrocytes or oligodendrocytes, isolated from mice treated or not with cuprizone for 5 weeks. We used Nucleospin RNA XS kit (Macherey Nagel, France) in accordance with the manufacturer’s protocol. The quality and quantity of each RNA preparation was assessed on the Agilent 2100 Bioanalyzer with RNA 6000 PicoChips (Agilent Technologies).
|
| Label |
Cy3
|
| Label protocol |
Ten nanograms of each RNA were amplified by IVT and labeled with Cy3 using the Low Imput Quick Amp labeling kit (Agilent Technologies) according to the manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
Five hundred nanogams of each Cy3-cRNA were hybridized overnight at 65°C on Whole Mouse Genome Microarray 4x44K v2 (Agilent Technologies) according to the manufacturer’s instructions.
|
| Scan protocol |
Microarrays data were acquired on an InnoScan 900 (Innopsys, France) with a resolution of 5 µm
|
| Data processing |
We scanned the chips using Nimbile Gem MS 200 (Roche).
Raw data were obtained with Mapix 5.0.0 software (Innopsys, France). For each sample, raw data consisted of the Median Feature Intensity – Median Background Intensity (F-B) at wavelength 532nm.
We then used R to normalize our data.
|
| |
|
| Submission date |
Jul 15, 2013 |
| Last update date |
Nov 06, 2014 |
| Contact name |
Sarah Moyon |
| E-mail(s) |
[email protected]
|
| Organization name |
CUNY ASRC
|
| Department |
Neuroscience
|
| Lab |
Casaccia
|
| Street address |
85 St Nicholas Terrace
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10031 |
| Country |
USA |
| |
|
| Platform ID |
GPL11202 |
| Series (1) |
| GSE48872 |
After demyelination in the adult CNS, oligodendrocyte precursor cells revert to an immature mRNA profile and express immune cues favoring their migration |
|
