|
| Status |
Public on Jul 01, 2013 |
| Title |
picogram 1 |
| Sample type |
SRA |
| |
|
| Source name |
H3K27me3 chromatin from embryonic nuclei
|
| Organism |
Drosophila melanogaster |
| Characteristics |
replicate: 1
chip antibody: H3K27me3
tissue: embryo
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
crosslinked nuclei were isolated from Drosophila embryos. 100 000 nuclei were treated with micrococcal nuclease to fragment the chromatin, and subjected to K27me3 ChIP by standard methods.
crosslinked nuclei were isolated from Drosophila embryos. 100 000 nuclei were treated with micrococcal nuclease to fragment the chromatin, and subjected to K27me3 ChIP by standard method (described in Bowman et al., BMC Genomics 2013)
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
K27me3 chromatin
|
| Data processing |
Base calling: Illumina
Alignment: Bowtie
Genome_build: dm3
Supplementary_files_format_and_content: The processed files give the counts of the aligned and filtered tags at the corresponding genomic positions in the following format:
Supplementary_files_format_and_content: chromosome, strand, coordinate of the tag 5'-end, tag count
|
| |
|
| Submission date |
Jun 30, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Sarah Keating Bowman |
| E-mail(s) |
[email protected]
|
| Organization name |
Massachusetts General Hospital
|
| Department |
Molecular Biology
|
| Street address |
185 Cambridge St
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02144 |
| Country |
USA |
| |
|
| Platform ID |
GPL13304 |
| Series (1) |
| GSE48431 |
Multiplexed Illumina sequencing libraries from picogram quantities of DNA |
|
| Relations |
| BioSample |
SAMN02221892 |
| SRA |
SRX316947 |
