|
| Status |
Public on Jul 01, 2015 |
| Title |
Prostate cancer cells, PC0251 |
| Sample type |
RNA |
| |
|
| Source name |
Laser micro dissected prostate tumor tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: prostate tumor
gleason_grade: 5
t-stage: pT3a
margin_status: Positive
preop_psa: 25.7
bcr: YES
bcr_free_time: 15
age: 59
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was performed using RNeasy Micro kit from Qiagen (Germany).
|
| Label |
biotin
|
| Label protocol |
A total of 5ng RNA was amplified and converted to cDNA using Nugen Pico-RNA system. After amplification, the cDNA was fragmented and labeled using NuGen FL-Ovation kit.
|
| |
|
| Hybridization protocol |
The labeled cDNA was loaded onto the Affymetrix U133 Plus 2.0 GeneChip according to the manufacturer's protocol.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix 3000 7G Scanner.
|
| Description |
PC0251
|
| Data processing |
Data was RMA normalized and intensity measures generated using GeneSpring version 11 software.
|
| |
|
| Submission date |
May 02, 2013 |
| Last update date |
Jul 01, 2015 |
| Contact name |
Martin Mørck Mortensen |
| E-mail(s) |
[email protected]
|
| Organization name |
Aarhus University Hospital
|
| Department |
Dept. of Urology
|
| Street address |
Brendstrupgaardsvej 100
|
| City |
Aarhus N |
| ZIP/Postal code |
DK-8200 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE46602 |
Expression data from prostate cancer and benign prostate glands |
|
