|
| Status |
Public on Jun 07, 2006 |
| Title |
MNG-22 (14 day hindlimb suspended rats treated with ALCAR)_HS+ALCAR D |
| Sample type |
RNA |
| |
|
| Source name |
soleus muscle, 3-month-old female pathogen-free Wistar rats
|
| Organism |
Rattus norvegicus |
| Characteristics |
Extracted molecule total RNA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from pooled 25 μm-thick cryostat sections of the muscle using modified RNeasy mini-protocol for skeletal muscle (Qiagen) as described in Wittwer et al., FASEB J 2002 Jun;16(8):884-6.
|
| Label |
P32
|
| Label protocol |
3 μg of isolated RNA was reverse-transcribed using the specific primers supplied with AtlasTM Rat 1.2 cDNA arrays (Clontech Laboratories) and 200 units of Superscript III reverse transcriptase (Invitrogen).
|
| |
|
| Hybridization protocol |
According the instructions supplied with AtlasTM Rat 1.2 cDNA arrays (Clontech Laboratories).
|
| Scan protocol |
The raw signal and local background of each probe spot on the membrane, given as the sum of pixels, was estimated with AIDA Array Easy software (Raytest Schweiz A.G., Urdorf, Switzerland) using the “local dot ring” mode as described in Wittwer et al., FASEB J 2002 Jun;16(8):884-6.
|
| Description |
Acetyl-L-carnitine (ALCAR)-treated 14-day suspended rats.RNA was isolated from cryosections using RNeasy kit (Qiagen). 3 microgram total RNA was subjected to microarray analysis.
|
| Data processing |
HS+ALCAR D; the data are normalized vs. the total sum of signals.
|
| |
|
| Submission date |
Jun 05, 2006 |
| Last update date |
Jan 11, 2008 |
| Contact name |
Maria Nicola Gadaleta |
| E-mail(s) |
[email protected]
|
| Phone |
+390805443309
|
| Fax |
+390805443403
|
| Organization name |
University of Bari, Italy
|
| Department |
Department of Biochemistry and Molecular Biology
|
| Street address |
via Orabona 4
|
| City |
Bari |
| ZIP/Postal code |
70125 |
| Country |
Italy |
| |
|
| Platform ID |
GPL153 |
| Series (1) |
| GSE4977 |
ALCAR supplementation to unloaded soleus muscle |
|
