|
| Status |
Public on Oct 20, 2013 |
| Title |
GFI1_knockout, rep2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
ILC2 cells, Gfi1 KO
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Gfi1 knockout
cell type: ILC2
|
| Growth protocol |
Cells were isolated from either wild-type or GFi1 KO mice. Purified ILC2s were cultured for 6 days in RPMI 1640, 10% FBS, Gln, P/S, 2ME, IL-2 (50ng/ml), IL-7 (10ng/ml), and IL-25 (50ng/ml).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Kit (Qiagen).
|
| Label |
Cy5
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Agilent protocol.
|
| |
|
| Channel 2 |
| Source name |
Stratagene Universal Mouse Reference RNA
|
| Organism |
Mus musculus |
| Characteristics |
sample type: reference
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Stratagene Universal Mouse Reference RNA (catalog number 740100).
|
| Label |
Cy3
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Agilent protocol.
|
| |
|
| |
| Hybridization protocol |
cRNA was hybridized on Agilent-026655 Whole Mouse Genome Microarray 4x44K.
|
| Scan protocol |
Chips were scanned using the Agilent Surescan Microarray Scanner.
|
| Description |
SAM9259612
Mouse GFi1 knockout ILC2 cells.
|
| Data processing |
The data were processed with Agilent Feature Extraction software, version 10.7.3.1, using default settings, including linear and Lowess normalization.
|
| |
|
| Submission date |
Mar 28, 2013 |
| Last update date |
Oct 20, 2013 |
| Contact name |
Alex Abbas |
| E-mail(s) |
[email protected]
|
| Organization name |
Genentech, Inc.
|
| Department |
Bioinformatics
|
| Street address |
1 DNA Way
|
| City |
South San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94080 |
| Country |
USA |
| |
|
| Platform ID |
GPL11202 |
| Series (1) |
| GSE45621 |
Mouse ILC2 cells from wild-type or Gfi1-KO animals |
|
