|
| Status |
Public on Feb 23, 2008 |
| Title |
L. lactis_30 min isoleucine starvation_ILV0.1 medium_replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
L. lactis IL1403 30 min isoleucine starvation
|
| Organism |
Lactococcus lactis |
| Characteristics |
strain IL1403
30 min isoleucine starvation
|
| Growth protocol |
see manuscript Redon et al. (in preparation). Comparative transcriptomic analysis of isoleucine starvation, CodY regulon and stringent response in Lactococcus lactis.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
see Redon et al. 2005. Role of mRNA stability during genome-wide adaptation of Lactococcus lactis to carbon starvation. J Biol Chem 280(43):36380-385.
|
| Label |
33P
|
| Label protocol |
see Redon et al. 2005. Role of mRNA stability during genome-wide adaptation of Lactococcus lactis to carbon starvation. J Biol Chem 280(43):36380-385.
|
| |
|
| Hybridization protocol |
see Redon et al. 2005. Role of mRNA stability during genome-wide adaptation of Lactococcus lactis to carbon starvation. J Biol Chem 280(43):36380-385.
|
| Scan protocol |
see Redon et al. 2005. Role of mRNA stability during genome-wide adaptation of Lactococcus lactis to carbon starvation. J Biol Chem 280(43):36380-385.
|
| Description |
Local background was removed from the raw signals with the Bioplot software (Biochips Platform, INSA Toulouse, France). Spots with intensities below the detection threshold were discarded. Detection threshold was defined as the mean intensity plus two standard deviations of empty spots. Data were normalized by the all spots mean with Bioplot software.
|
| Data processing |
Local background was removed from the raw signals with the Bioplot software (Biochips Platform, INSA Toulouse, France). Spots with intensities below the detection threshold were discarded. Detection threshold was defined as the mean intensity plus two standard deviations of empty spots. Data were normalized by the all spots mean with Bioplot software.
|
| |
|
| Submission date |
May 18, 2006 |
| Last update date |
Feb 23, 2007 |
| Contact name |
Muriel Cocaign-Bousquet |
| E-mail(s) |
[email protected]
|
| URL |
http://www.insa-toulouse.fr/fr/recherche/labo/lisbp.html
|
| Organization name |
Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés
|
| Department |
UMR5504 INSA/CNRS - UMR792 INSA/INRA
|
| Street address |
135 avenue de Rangueil
|
| City |
Toulouse |
| ZIP/Postal code |
31077 |
| Country |
France |
| |
|
| Platform ID |
GPL3782 |
| Series (1) |
| GSE4872 |
Response of Lactococcus lactis to isoleucine starvation |
|
