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| Status |
Public on Feb 23, 2008 |
| Title |
Response of Lactococcus lactis to isoleucine starvation |
| Organism |
Lactococcus lactis |
| Experiment type |
Expression profiling by array
|
| Summary |
For the first time in Lactococcus lactis, amino acid starvation response was characterized. The natural imposition of isoleucine starvation, by its consumption during growth, associated to transcript profiling, allowed defining exhaustively this stress stimulon. It consisted of a general induction of nitrogen metabolism (amino acid biosynthesis and transport, proteolytic system and proteases), a strong repression of genes encoding major physiological activities (translation, transcription, carbon metabolism, purine and pyrimidine biosynthesis and fatty acid metabolism) and the induction of unexpected cross responses to acid, osmotic and oxidative stresses.
Keywords: stress response, time course
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| Overall design |
Isoleucine starvation was imposed by the consumption of this amino acid during the growth of Lactococcus lactis IL1403 on ILV0.1 medium (CDM with ten-fold reduced concentrations of isoleucine, leucine and valine) and under controlled conditions (30 °C, pH 6.6, nitrogen atmosphere). Cell samples were harvested in exponential phase and after 30 min, 1.7 h and 3.5 h of isoleucine starvation. Total RNA was extracted from these samples and radiolabelled cDNA were prepared and hybridized on nylon arrays. 2053 amplicons specific of Lactococcus lactis IL1403 genes were spotted twice on the array. The 4 time-points were analyzed simultaneously and 3 independent repetitions were performed.
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| Contributor(s) |
Redon E, Cocaign-Bousquet M, Loubiere P |
| Citation(s) |
18644113 |
| |
| Submission date |
May 19, 2006 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Muriel Cocaign-Bousquet |
| E-mail(s) |
[email protected]
|
| URL |
http://www.insa-toulouse.fr/fr/recherche/labo/lisbp.html
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| Organization name |
Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés
|
| Department |
UMR5504 INSA/CNRS - UMR792 INSA/INRA
|
| Street address |
135 avenue de Rangueil
|
| City |
Toulouse |
| ZIP/Postal code |
31077 |
| Country |
France |
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| Platforms (1) |
| GPL3782 |
INSA Lactococcus lactis IL1403 (lot de PCR n°1) |
|
| Samples (12)
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| GSM109311 |
L. lactis_exponential phase_ILV0.1 medium_replicate 1 |
| GSM109314 |
L. lactis_exponential phase_ILV0.1 medium_replicate 2 |
| GSM109315 |
L. lactis_exponential phase_ILV0.1 medium_replicate 3 |
| GSM109316 |
L. lactis_30 min isoleucine starvation_ILV0.1 medium_replicate 1 |
| GSM109317 |
L. lactis_30 min isoleucine starvation_ILV0.1 medium_replicate 2 |
| GSM109318 |
L. lactis_30 min isoleucine starvation_ILV0.1 medium_replicate 3 |
| GSM109319 |
L. lactis_1.7 h isoleucine starvation_ILV0.1 medium_replicate 1 |
| GSM109320 |
L. lactis_1.7 h isoleucine starvation_ILV0.1 medium_replicate 2 |
| GSM109321 |
L. lactis_1.7 h isoleucine starvation_ILV0.1 medium_replicate 3 |
| GSM109322 |
L. lactis_3.5 h isoleucine starvation_ILV0.1 medium_replicate 1 |
| GSM109324 |
L. lactis_3.5 h isoleucine starvation_ILV0.1 medium_replicate 2 |
| GSM109325 |
L. lactis_3.5 h isoleucine starvation_ILV0.1 medium_replicate 3 |
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| Relations |
| BioProject |
PRJNA95935 |
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