|
| Status |
Public on Mar 22, 2024 |
| Title |
Moderate Responder 4 to Etanercept |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
PBMCs
|
| Organism |
Homo sapiens |
| Characteristics |
gender: F
cell type: PBMC
disease state: rheumatoid arthritis
treatment: methotrexate + etanercept
efficacy: moderate response
|
| Treatment protocol |
PBMCs from total blood for each patient were isolated by Ficoll and preserved in RNA later at -20°C until total RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from PBMC were extracted using Trizol following manufacturer's instructions.
|
| Label |
Cy5
|
| Label protocol |
Agilent QuickAmp Labeling kit was used according to the manufacturer instructions (catalog number 5190-0443) with 500ng of total RNA.
|
| |
|
| Channel 2 |
| Source name |
PBMCs from 10 healthy donnors
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: healthy
cell type: PBMC
sample type: Pool from 5 women and 5 men
|
| Treatment protocol |
PBMCs from total blood for each patient were isolated by Ficoll and preserved in RNA later at -20°C until total RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from PBMC were extracted using Trizol following manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Agilent QuickAmp Labeling kit was used according to the manufacturer instructions (catalog number 5190-0443) with 500ng of total RNA.
|
| |
|
| |
| Hybridization protocol |
Hybridization was performed according to the Agilent instructions for Gene Expression Hybridization Kit (catalog number 5188-5242). Samples were applied to microarrays enclosed in Agilent SureHyb hybridization chambers (65°C, 17H). After hybridization, slides were washed and dried in acetonitril.
|
| Scan protocol |
Scanned on an Agilent G2565CA scanner. Images were quantified using Agilent Feature Extraction Software (v 10.5.1.1)
|
| Data processing |
Agilent Feature Extraction Software (v 10.5.1.1) was used for LOWESS normalization.
|
| |
|
| Submission date |
Dec 11, 2012 |
| Last update date |
Mar 22, 2024 |
| Contact name |
Celine Derambure |
| E-mail(s) |
[email protected]
|
| Organization name |
INSERM U905
|
| Street address |
22 Bvd Gambetta
|
| City |
Rouen cedex |
| ZIP/Postal code |
76183 |
| Country |
France |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
| GSE42842 |
The predictive biomarkers of TNFα-blocking agent responsiveness are specific for adalimumab and etanercept |
|
