|
| Status |
Public on Oct 31, 2013 |
| Title |
Sarcoid_PBMC Sample 65 cell separation |
| Sample type |
RNA |
| |
|
| Source name |
PBMC
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: Sarcoid
cell type: PBMC
|
| Treatment protocol |
Whole blood stored in Tempus, PBMCs obtained using Lymphoprep then leucocytes purified using Miltenyi whole blood MACS beads, neutrophils obtained after Lymphoprep and red blood cell lysis then Miltenyi MACS beads
|
| Growth protocol |
Direct ex vivo human blood frozen and purified leucocytes after RNA extraction frozen
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Qiagen RNeasy Mini Kit.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated, amplified antisense cRNA were prepared using NuGEN WT-Ovation RNA Amplification and Encore BiotinIL Module.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
Background subtracted, normalised and log2 transformed for normalised values and non-normalised values have only had background subtraction
|
| |
|
| Submission date |
Dec 10, 2012 |
| Last update date |
Oct 31, 2013 |
| Contact name |
Chloe Bloom |
| Organization name |
MRC National Institute for Medical Research
|
| Street address |
The Ridgeway
|
| City |
London |
| ZIP/Postal code |
NW7 1AA |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE42832 |
Microarray study using whole blood and purified leucocyte populations from healthy controls and patients with tuberculosis or sarcoidosis with tuberculosis and sarcoidosis |
| GSE42834 |
Human whole blood microarray study to compare patients with tuberculosis, sarcoidosis, pneumonia, and lung cancer |
|
