|
| Status |
Public on Oct 31, 2013 |
| Title |
Sarcoid Sample 31 validation set |
| Sample type |
RNA |
| |
|
| Source name |
Whole Blood
|
| Organism |
Homo sapiens |
| Characteristics |
gender: M
ethnicity: Black
disease state: Non-active sarcoidosis
tissue: whole blood
|
| Treatment protocol |
Direct ex-vivo human blood
|
| Growth protocol |
Blood was taken into Tempus and frozen
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNon-active sarcoidosis was extracted using the MagMAX-96 Blood RNon-active sarcoidosis Isolation Kit and globin reduced using GLOBINclear
|
| Label |
biotin
|
| Label protocol |
Biotinylated, amplified cRNon-active sarcoidosis were prepared using the IllumiNon-active sarcoidosis CustomPrep RNon-active sarcoidosis amplification kit
|
| |
|
| Hybridization protocol |
Standard IllumiNon-active sarcoidosis hybridization protocol
|
| Scan protocol |
Standard IllumiNon-active sarcoidosis scanning protocol
|
| Data processing |
The data were normalised using 75th percentile normalisation in GeneSpring 11.5
Background subtracted, normalised and log2 transformed for normalised values and non-normalised values have only had background subtraction
|
| |
|
| Submission date |
Dec 10, 2012 |
| Last update date |
Oct 31, 2013 |
| Contact name |
Chloe Bloom |
| Organization name |
MRC National Institute for Medical Research
|
| Street address |
The Ridgeway
|
| City |
London |
| ZIP/Postal code |
NW7 1AA |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE42825 |
Human whole blood microarray study to compare patients with tuberculosis, sarcoidosis, pneumonia, and lung cancer (validation) |
| GSE42834 |
Human whole blood microarray study to compare patients with tuberculosis, sarcoidosis, pneumonia, and lung cancer |
|
