|
| Status |
Public on Jun 30, 2013 |
| Title |
late alcohol drinker-2 |
| Sample type |
RNA |
| |
|
| Source name |
Hypo11
|
| Organism |
Mus musculus |
| Characteristics |
group: late alcohol drinker
strain: ICR
tissue: Hypothalamus
gender: Male
|
| Treatment protocol |
On day 51 of the experiment, each mouse is sacrificed by decapitation, and their hypothalami were quickly removed and frozen in N-hexane (−70 °C) for approximately 40 s. The samples were then stored at −80 ◦C until further use.
|
| Growth protocol |
Before chronic alcohol drinking began, mice (3 weeks old) were allowed to adapt to drinking tubes with both tubes containing water from experimental day 1-5. After adaptation period, mice were randomly aside to 5% alcohol group, 10% alcohol group or water-only control group (n=16-18). For the water-only control group, two drinking tubes in each cage contained deionized water throughout the duration of the experiment. For the alcohol group, one drinking tube contained 5% or 10% alcohol solution while the other contained water.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the TRIzol reagent (Invitrogen, Carlsbad, CA, USA) and purified with RNeasy column (Qiagen, Valencia, CA).The RNA concentration and purity were analyzed by a Nanodrop spectrophotometer (Nanodrop technologies, Wilmington, DE), with the spectral absorption at 260 and 280 nm. The assessment of the RNA integrity was conducted by the Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA). All RNA samples had RNA Integrity Numbers (RIN) of 8.0 or higher.
|
| Label |
Cy3
|
| Label protocol |
Total RNA was Cy3 labelled according to Agilent’s Low RNA Input Fluorescent Linear Amplification Kit.
|
| |
|
| Hybridization protocol |
Hybridized onto Agilent Whole Mouse Genome 4 × 44K G4122F microarrays containing 43 604 probes as described in the manufacturer's protocol.
|
| Scan protocol |
Slides were scanned (Agilent G2505B) at 5 μm resolution using an extended dynamic range protocol, and images were processed with Agilent Feature Extraction software 9.5.
|
| Description |
Hypothalamic gene expression after chronic alcohol consumption from adolescence-to-adulthood in mice, which was a late high alcohol drinker
|
| Data processing |
Within-array normalization was performed using the “Background detrending” software (Agilent). The nonuniform outlier features (spots) were removed.
|
| |
|
| Submission date |
Dec 06, 2012 |
| Last update date |
Jun 30, 2013 |
| Contact name |
ke wang |
| E-mail(s) |
[email protected]
|
| Phone |
+86 21 51320288
|
| Organization name |
Shanghai Biochip Co., Ltd.
|
| Street address |
151 Libing Road
|
| City |
Shanghai |
| ZIP/Postal code |
201203 |
| Country |
China |
| |
|
| Platform ID |
GPL7202 |
| Series (1) |
| GSE42770 |
Chronic alcohol consumption from adolescence-to-adulthood in mice — Effect on gene expression in the hypothalamus |
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