|
| Status |
Public on Oct 27, 2012 |
| Title |
2A3-3-asTF, replicate3 |
| Sample type |
RNA |
| |
|
| Source name |
2A3-3-asTF
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF-7-based 2A3-3
cell type: breast cancer
genotype/variation: alternatvively spliced tissue factor (asTF)
|
| Growth protocol |
Cells were seeded in 6-wells plates and grown for three days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Nucleospin II (Macherey-Nagel, Düren, Germany) according to the manufacturer’s protocol.
|
| Label |
biotin
|
| Label protocol |
RNA was amplified and labeled using Illumina TotalprepTM -96 RNA Amplification Kit (Ambion, #4393543)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 3
|
| Data processing |
The data were normalised by using loess normalisation via a Lumi library
|
| |
|
| Submission date |
Oct 26, 2012 |
| Last update date |
Oct 27, 2012 |
| Contact name |
Henri H Versteeg |
| E-mail(s) |
[email protected]
|
| Phone |
+31-71-5263872
|
| Organization name |
Leiden University Medical Center
|
| Department |
Einthoven Lab for Experimental Vascular Medicine
|
| Street address |
Albinusdreef 2
|
| City |
Leiden |
| ZIP/Postal code |
2333 ZA |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE41872 |
Gene expression analysis after selective expression of tissue factor isoforms |
|
