|
| Status |
Public on Sep 06, 2013 |
| Title |
Sema NRP KO gmd056 |
| Sample type |
RNA |
| |
|
| Source name |
murine T cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6
treatment: Sema
genotype: Nrp fl/fl x Foxp3-YFP-iCre
|
| Treatment protocol |
Immobilized anti-CD3 (1 µg/mL), soluble anti-CD28 (2 µg/mL), rhIL-2 (100 U/mL), immobilized mouse IgG1 or Sema4a-Ig (10 µg/mL
|
| Growth protocol |
Flow cytometric sorting of CD4+CD45RBloFoxp3-YFP+ cells from bulk lymphocytes (spleen and peripheral LN)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TriZOL extraction with 1 µL glycogen as a carrier
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the Affymetrix 3' IVT Express protocol
|
| |
|
| Hybridization protocol |
Following fragmentation, 5 microgram of cRNA was hybridized for 16 hr at 45C on each array. GeneChips were washed and stained in the Affymetrix GeneTitan system.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneTitan system.
|
| Description |
CD4+CD45RBloFoxp3-YFP+ regulatory T cells
|
| Data processing |
data was RMA summarized, QC checked by PCA , and batch corrected in Partek Genomics Suite 6.6
|
| |
|
| Submission date |
Sep 27, 2012 |
| Last update date |
Sep 06, 2013 |
| Contact name |
David Finkelstein |
| E-mail(s) |
[email protected]
|
| Phone |
9014953931
|
| Organization name |
St Jude Children's Research Hospital
|
| Department |
Computational Biology
|
| Street address |
332 N. Lauderdale St.
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL11180 |
| Series (1) |
| GSE41185 |
Potentiation of regulatory T cell stability and function via a neuropilin-1:semaphorin-4a axis |
|
