|
| Status |
Public on Sep 06, 2013 |
| Title |
Treg activated cjw185 |
| Sample type |
RNA |
| |
|
| Source name |
bone marrow
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6
genotype: Foxp3GFP
|
| Treatment protocol |
CD4+ T cells were left untreated (samples cjw 173, 183, 193, 186, 196) or were activated with anti-CD3/CD28
|
| Growth protocol |
CD4+ T cells (subtype indicated above) were cultured in RPMI media, supplemented with 10% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Rneasy Plus Mini kit was used to extract RNA according to the manufacturer's protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix Two-Cycle protocol from 1 ug of total RNA (Expression Analysis Technical Manual, 2005-2006, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
| Description |
CD4+/CD45RBlow/GFP+ cells were purified by FACS and cultured with stimulation for 48 hours
|
| Data processing |
data was RMA summarized, QC checked by PCA , and batch corrected in Partek Genomics Suite 6.6
|
| |
|
| Submission date |
Sep 27, 2012 |
| Last update date |
Sep 06, 2013 |
| Contact name |
David Finkelstein |
| E-mail(s) |
[email protected]
|
| Phone |
9014953931
|
| Organization name |
St Jude Children's Research Hospital
|
| Department |
Computational Biology
|
| Street address |
332 N. Lauderdale St.
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE41185 |
Potentiation of regulatory T cell stability and function via a neuropilin-1:semaphorin-4a axis |
|
