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| Status |
Public on Feb 15, 2018 |
| Title |
ChIP-Seq of H3K9me3 and H3K36me3 after GFP or dKDM4A RNAi of S2 cells |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Analysis of H3K9me3 and H3K36me3 levels in unique and repetitive regions of Drosophila genome after GFP (control) or dKDM4A RNAi of S2 cells
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| Overall design |
After 5 days of RNAi, chromatin was prepared by fixation of 2x10^7 S2 cells with 1% paraformaldehyde for 10 minutes and shearing with Bioruptor sonicator (30 cycles). ChIP was performed by overnight incubation of chromatin with Protein-A Dynabeads and 5 ug of either anti-H3K36me3 or anti-H3K9me3 ChIP-grade antibody (Abcam). Library construction was conducted using TruSeq DNA sample preparation kit (Illumina). All sequencing data were obtained from the Vincent J. Coates Genomics Sequencing Laboratory at UC Berkeley.
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| Contributor(s) |
Colmenares SU, Karpen GH |
| Citation(s) |
28743002 |
| |
| Submission date |
May 17, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
serafin colmenares |
| E-mail(s) |
[email protected]
|
| Organization name |
lawrence berkeley national lab
|
| Street address |
1 cyclotron road MS977R180A
|
| City |
berkeley |
| ZIP/Postal code |
94720 |
| Country |
USA |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (12)
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| GSM2630590 |
GFPRNAi_H3K36Me3ChIP3.filtered.fastq.gz |
| GSM2630591 |
GFPRNAi_H3K36Me3ChIP4_filtered.fastq.gz |
| GSM2630592 |
GFPRNAi_H3K9Me3ChIP3_filtered.fastq.gz |
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| Relations |
| BioProject |
PRJNA387028 |
| SRA |
SRP107219 |
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