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| Status |
Public on Feb 06, 2018 |
| Title |
Gene expression in mammary glands of lactating wild-type or Th-POK-deficient mice. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The Th-inducing POK (Th-POK, also known as cKrox or ZBTB7B) transcription factor is a key regulator of lineage commitment of immature T cell precursors. It is yet unclear the physiological functions of Th-POK besides helper T cell differentiation. We found that Th-POK is restrictedly expressed in the luminal epithelial cells in the mammary glands. Th-POK is not required for mammary gland development in puberty and alveologenesis in pregnancy. Th-POK-deficient mice are defective in secretory activation upon parturition with large cellular lipid droplets retained within alveolar epithelial cells. We compared microarray gene expression in mammary glands of lactating wild-type or Th-POK-deficient mice.
We show that Th-POK directly regulates expression of insulin receptor substrate-1 (IRS-1) and insulin-induced Akt-mTOR-SREBP signaling. Th-POK deficiency compromises IRS-1 expression and Akt-mTOR-SREBP signaling in the mammary glands. Thus, Th-POK function as an important regulator of insulin signaling in mammary gland lactation.
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| Overall design |
No. 4 mammary glands were harvested and snap frozen from quadruplicate wild-type or Th-POK-deficient dams at lactation day 1. Total RNA was extracted from the mammary glands for microarray analyses (Agilent Whole Mouse Genome Microarray 4x44K array, Design ID: 014868) at the Shanghai Biotech Corp., following the manufacturer's recommendation.
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| Contributor(s) |
Zhang R, Ma H, Ge G |
| Citation(s) |
29420538 |
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| Submission date |
Apr 10, 2017 |
| Last update date |
Mar 07, 2018 |
| Contact name |
Gaoxiang Ge |
| E-mail(s) |
[email protected]
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| Phone |
86-21-54921105
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| Organization name |
Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences
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| Street address |
320 Yue-Yang Rd
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| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA382330 |
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