|
| Status |
Public on Sep 12, 2018 |
| Title |
Next Generation Sequencing Analysis of gene expression profile in E9.5 Mesp1Cre/+/Dgcr8-/- embryonic heart cells transfected with NC miRNA and miR-541 mimics |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: To compare the E9.5 Dgcr8 conditional knockout embryonic heart cells transfected with NC miRNA and miR-541 mimics
Methods: In vitro cultured E9.5 Dgcr8 conditional KO heart cells transfected with miR-541-5p and NC miRNA were extracted with TRIZOL 48hrs after transfection, and 10ng total RNA was reverse transcribed and amplified by Smart-seq2 protocol as described (Picelli et al., 2014). Duplicated biological samples were analyzed using Illumina HiSeqX10, Clean reads were mapped to mouse genome (mm9) using BWA software.
Results: Genes differentially expressed in E9.5 Dgcr8 cKO embryonic heart cells transfected with NC miRNA and miR-541 were identified.
Conclusions: miRNA-541 significantly changes the gene expression profiles of E9.5 Dgcr8 cKO embryonic heart cells and promote the cardiac function
|
| |
|
| Overall design |
Perform RNAseq of E9.5 Dgcr8 cKO embryonic heart cells transfected with NC miRNA and miR-541 mimics
|
| |
|
| Citation(s) |
30128894 |
| |
| Submission date |
Mar 15, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Jie Na |
| E-mail(s) |
[email protected]
|
| Phone |
+86-18618192150
|
| Organization name |
Tsinghua University
|
| Department |
Basic Medical Sciences, School of Medicine
|
| Lab |
D115 School of Medicine
|
| Street address |
Tsinghua University
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100084 |
| Country |
China |
| |
|
| Platforms (1) |
|
| Samples (4)
|
|
| This SubSeries is part of SuperSeries: |
| GSE96648 |
Transcriptome analysis of mouse primitive heart with Dgcr8 deletion |
|
| Relations |
| BioProject |
PRJNA379286 |
| SRA |
SRP101947 |
Less...
More...