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| Status |
Public on Oct 01, 2017 |
| Title |
Identification of differential expressed genes of JQ1 or JQ1+Bortezomib in colorectal cancer cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The bromodomain and extra-terminal domain inhibitors (BETi) are promising epigenetic drugs for the treatment of various cancers through suppression of oncogenic transcription factors including MYC. However, only a subset of CRC cells response to BETi, suggesting an intrinsic resistance to BETi in CRC. We investigated the effect of JQ1 on cell proliferation, apoptosis, angiogenesis and MYC expression in a panel of 11 CRC cells in vitro and in vivo. JQ1-resistant CRC cells were used for the screening for the effective combination therapies with JQ1. RNA-seq of single drug or combined drugs treatment was performed to explore the mechanism of action.
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| Overall design |
RKO cells were treated with JQ1 (1 μM) for 6 h. HCT116 cells were treated with DMSO, JQ1 (1 μM) , Bortezomib (5nM), JQ1 (1 μM)+Bortezomib (5nM) for 6h. RNA was extracted for RNA-seq.
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| Contributor(s) |
Liu C, Cui L |
| Citation(s) |
29472532 |
| |
| Submission date |
Feb 28, 2017 |
| Last update date |
Oct 31, 2022 |
| Contact name |
Chen-Ying Liu |
| E-mail(s) |
[email protected]
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| Organization name |
Xinhua Hospital, Shanghai Jiao Tong University
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| Street address |
1665 Kong Jiang Road
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| City |
Shanghai |
| ZIP/Postal code |
200092 |
| Country |
China |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA377308 |
| SRA |
SRP100857 |
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