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| Status |
Public on Jul 31, 2021 |
| Title |
The Trithorax group protein ASH1 requires a combination of BAH domain and AT hooks, but not the SET domain, for mitotic chromatin binding and survival |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The Drosophila Trithorax group (TrxG) protein ASH1 remains associated with mitotic chromatin through mechanisms that are poorly understood. ASH1 dimethylates histone H3 at lysine 36 via its SET domain. Here we identify domains of the TrxG protein ASH1 that are required for mitotic chromatin attachment in living Drosophila. Quantitative live imaging demonstrates that ASH1 requires AT hooks and the BAH domain but not the SET domain for full chromatin binding in metaphase, and that none of these domains are essential for interphase binding. Genetic experiments show that disruptions of the AT hooks and the BAH domain together, but not deletion of the SET domain alone, are lethal. Transcriptional profiling demonstrates that intact ASH1 AT hooks and the BAH domain are required to maintain expression levels of a specific set of genes, including several involved in cell identity and survival. This study identifies in vivo roles for specific ASH1 domains in mitotic binding, gene regulation and survival that are distinct from its functions as a histone methyltransferase.
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| Overall design |
Transgenic flies expressing variants of the ASH1 protein fused to EGFP in an ash1 mutant background, were generated. RNAseq analysis was performed on 3rd instar larval wing discs of each of three variants, namely EGFP::ASH1 WT, EGFP::ASH1DBAH and EGFP::ASH1DBAH mut AT1 and compared to wing discs from wild type flies.
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| Contributor(s) |
Ringrose L, Juntilla S |
| Citation(s) |
34331109 |
| |
| Submission date |
Feb 22, 2017 |
| Last update date |
Aug 01, 2021 |
| Contact name |
Leonie Ringrose |
| E-mail(s) |
[email protected]
|
| Phone |
+ 49 30 2093 49772
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| Organization name |
Humboldt University Berlin
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| Department |
IRI Lifesciences
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| Lab |
Haus 22
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| Street address |
Philippstr. 13
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| City |
Berlin |
| ZIP/Postal code |
10115 |
| Country |
Germany |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA376442 |
| SRA |
SRP100568 |
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