|
| Status |
Public on Nov 16, 2016 |
| Title |
Master Regulators of oncogenic KRAS response in Pancreatic Cancer |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We derived a transcriptional signature of oncogenic KRAS by using the KF508 murine pancreatic ductal cell line with an inducible Lox-Stop-Lox (LSL) cassette in front of the KRASG12D oncogene to regulate transcription. This dataset allowed us to study the differential expression profile after oncogenic KRAS induction in mouse.
|
| |
|
| Overall design |
Total RNA was extracted from six sets of mock and cre treated ductal cells using Qiagen RNeasy kit. The RNA was labelled and hybridized. This experiment was carried out on 2 Illumina Mousev2 BeadChips using 24 arrays (1 samples per Bead-Chip).
|
| |
|
| Contributor(s) |
de Santiago I, Sivakumar S, Chlon L, Markowetz F |
| Citation(s) |
28141826 |
| |
| Submission date |
Nov 15, 2016 |
| Last update date |
Nov 24, 2017 |
| Contact name |
Ines de Santiago |
| E-mail(s) |
[email protected]
|
| Phone |
07482446828
|
| Organization name |
University of Cambridge
|
| Department |
CRUK
|
| Street address |
Robinson Way
|
| City |
Cambridge |
| State/province |
- Select - |
| ZIP/Postal code |
CB1 2NR |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL17543 |
Illumina MouseWG-6 v2.0 R2 expression beadchip |
|
| Samples (24)
|
|
| Relations |
| BioProject |
PRJNA353591 |
Less...
More...