|
| Status |
Public on Nov 16, 2016 |
| Title |
KF508_Mock_6_2 |
| Sample type |
RNA |
| |
|
| Source name |
KF508 with inducible LSL cassette in front of KRASG12D oncogene, Mock treated
|
| Organism |
Mus musculus |
| Characteristics |
cell line: KF508 pancreatic ductal cell line
treatment: Mock
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Mock_6_Rep2
|
| Data processing |
The data were normalized using quantile normalisation in R. Differential expression analysis between the two groups (“Mock” vs “Cre”) was carried out using limma and the illuminaArrayPipeline. In addition, the limma function 'duplicateCorrelation' was to estimate the correlation between measurements made on the same technical replicate.
|
| |
|
| Submission date |
Nov 15, 2016 |
| Last update date |
Nov 16, 2016 |
| Contact name |
Ines de Santiago |
| E-mail(s) |
[email protected]
|
| Phone |
07482446828
|
| Organization name |
University of Cambridge
|
| Department |
CRUK
|
| Street address |
Robinson Way
|
| City |
Cambridge |
| State/province |
- Select - |
| ZIP/Postal code |
CB1 2NR |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL17543 |
| Series (1) |
| GSE89846 |
Master Regulators of oncogenic KRAS response in Pancreatic Cancer |
|
