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| Status |
Public on May 17, 2018 |
| Title |
RNA-seq analysis of transcriptomic changes caused by Egg/dSETDB1 in Drosophila |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Egg/dSETDB1, the ortholog of the human methyltransferase SETDB1, is the only H3K9 methyltransferase essential for viability and fertility in Drosopila. To understand the role of Egg in oogenesis, we adopted high throughput RNA sequencing (RNA-seq) and identified putative target genes regulated by Egg.
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| Overall design |
To obtain total RNAs and cDNAs, ovaries were dissected from 2- to 4-day-old wild type (w1118) or egg mutant (egg2138/Df(2R)Dll-Mp) females and frozen in liquid nitrogen.
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| Contributor(s) |
Yun LJ, Woongjoon M, Chanseok S |
| Citation(s) |
29740006 |
| |
| Submission date |
Sep 29, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Jae Yun Lim |
| E-mail(s) |
[email protected]
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| Phone |
82-2-880-4940
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| Organization name |
Seoul National University
|
| Street address |
1 Gwanak-ro, Gwanak-gu
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| City |
Seoul |
| ZIP/Postal code |
08826 |
| Country |
South Korea |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (2) |
| GSM2332554 |
Ovaries of Drosophila melanogaster strain w1118 |
| GSM2332555 |
Ovaries of Drosophila melanogaster egg2138/Df(2R)Dll-Mp mutants |
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| Relations |
| BioProject |
PRJNA344906 |
| SRA |
SRP090634 |
